Sbp (NM_011321) Mouse Tagged ORF Clone

CAT#: MG202009

Sbp (tGFP-tagged) - Mouse spermine binding protein (Sbp)

Product Images

Circular map for MG202009

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sbp
• Synonyms: AV082137; AV082259; p25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202009 representing NM_011321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTTGCTGACTTTGGCTTTCCTGGCCAGTCCCACATGCAGAGCCCAGAATGTCCTGGGGAATG
  CTGCTGGCAAGTATTTCTACGTTCAAGGTGAAGATCAGGGGCAACTCAAGGGCATGCGGATCTTCTTAAG
  TGTTTTCAAGTTCATCAAGGGCTTCCAGCTGCAGTTTGGCAGTAACTGGACTGATGTCTATGGAACCCGG
  TCAGATAACTTTATAGACTTCCTTCTGGAGGACGGAGAGCACGTGATAAAGGTAGAGGGCAGTGCAGTGA
  TCTGCCTGACTTCCCTGACCTTCACTACAAACAAGGGGCGTGTGGCCACCTTTGGTGTTAGAAGGGGTCG
  ATACTTCAGTGACACTGGAGGTTCAGACAAGCATCTAGTGACTGTCAATGGAATGCATGCACCGGGCCTC
  TGCGTCAGAGGGATTGGCTTCAAATGGGGAAATATCAATGCTAATGGCAATGACCATTATAATAACAAGG
  AAGATAAGGCTGACAACAAAGATGCTGACAACAAAGATGCTGACAACAAAGATGATGGAGATGAGGATGA
  TGATGGAAACGATGATGATGACCAGAAAGATGAAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202009 representing NM_011321
  Red=Cloning site Green=Tags(s)
  
  MLLLLTLAFLASPTCRAQNVLGNAAGKYFYVQGEDQGQLKGMRIFLSVFKFIKGFQLQFGSNWTDVYGTR
  SDNFIDFLLEDGEHVIKVEGSAVICLTSLTFTTNKGRVATFGVRRGRYFSDTGGSDKHLVTVNGMHAPGL
  CVRGIGFKWGNINANGNDHYNNKEDKADNKDADNKDADNKDDGDEDDDGNDDDDQKDES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011321
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011321.4
• RefSeq Size: 839 bp
• RefSeq ORF: 600 bp
• Locus ID: 20234
• UniProt ID: P15501
• Cytogenetics: 17 A3.3
• Gene Summary: This protein seems to be functional equivalent to rat prostatic spermine-binding protein, which is involved in polyamine binding.[UniProtKB/Swiss-Prot Function]