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Ak1 (BC014802) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201878
Ak1 (tGFP-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ak1 or BC014802"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ak1
Synonyms Ak-1; B430205N08Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201878 representing BC014802
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGAAGCTGAAGAAGGCCAAGATCATCTTTGTGGTGGGCGGACCTGGCTCAGGAAAGGGCACCC
AGTGCGAGAAGATTGTACAGAAATATGGCTACACCCACCTGTCTACTGGGGACCTGCTTCGGGCAGAAGT
CAGCTCTGGATCGGAGAGGGGCAAGAAGCTGTCGGCTATCATGGAGAAGGGAGAGCTAGTGCCACTGGAC
ACGGTGCTGGACATGCTCCGAGATGCTATGTTAGCCAAAGTGGATTCTTCCAATGGCTTCCTGATCGACG
GCTACCCGAGGGAGGTGAAACAGGGAGAAGAATTTGAACAGAAGATTGGACAGCCCACACTGCTGCTGTA
TGTGGACGCAGGCGCCGAGACCATGACCCAACGACTCCTGAAGCGAGGGGAGACCAGTGGCCGCGTGGAT
GACAACGAGGAGACCATCAAGAAGCGGCTGGAGACTTATTACAATGCCACAGAACCTGTCATCTCCTTCT
ATGACAAGCGTGGCATTGTGCGCAAGGTCAATGCCGAAGGCACGGTGGACACTGTCTTCTCTGAGGTCTG
CACCTATCTTGACTCCCTGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201878 representing BC014802
Red=Cloning site Green=Tags(s)

MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLD
TVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVD
DNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC014802
ORF Size 582 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC014802, AAH14802
RefSeq Size 2059 bp
RefSeq ORF 584 bp
Locus ID 11636
Cytogenetics 2 22.09 cM
Summary Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC014802" in other vectors (3)
SKU Description Size Price
MR201878 Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) 10 ug
$289.00 $300.00
MR201878L3 Lenti ORF clone of Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) 10 ug
$600.00
MR201878L4 Lenti ORF clone of Ak1 (mGFP-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.