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Ociad1 (BC006937) Mouse Tagged ORF Clone

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SKU
MG201797
Ociad1 (tGFP-tagged) - Mouse OCIA domain containing 1 (cDNA clone MGC:6810 IMAGE:2648369)
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  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ociad1
Synonyms OCIA, TPA018
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201797 representing BC006937
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGGAGGGCTGATTTTCGAGAACCGAATGCACAAGTGTCAAGACCTATTCCCGACATAGGAGGCG
GTTATATTCCGACAGAGGAGGAGTGGAGACTCTTTGCAGAGTGCCATGAAGAGTGCTTCTGGTTCAGATC
TGTGCCGTTGGCTGCCACAAGTATGCTGATTACTCAAGGATTAATTAGTAAAGGAATCCTGTCAAGTCAT
CCAAAATATGGTTCAATTCCTAAACTTCTATTCGCTTGTATCGTGGGGTACTTCGCTGGAAAGCTTTCTT
ACGTGAAAACGTGCCAAGAGAAGTTCAAGAAGCTTGAAAACTCCCCCCTTGGAGAAGCACTGCGGTCGGG
AGAATTGCGGCGCTCTTCTCCACCAGGGCACTATACTCAAAAGCCTAAATTTGACTCAAATGTGAGTGGT
CAGTCCTCTTTCGGGACATCTCCAGCAGCAGACAACATAGAAAAGGAGGCGCTTCCTCGTTATGAGCCAA
TTCCATTTAGTGCTTCTATGAACGAGTCCACTCCCACTGGTATTACTGACCATATTGCCCAAGGTAGAAA
CTTCTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201797 representing BC006937
Red=Cloning site Green=Tags(s)

MNGRADFREPNAQVSRPIPDIGGGYIPTEEEWRLFAECHEECFWFRSVPLAATSMLITQGLISKGILSSH
PKYGSIPKLLFACIVGYFAGKLSYVKTCQEKFKKLENSPLGEALRSGELRRSSPPGHYTQKPKFDSNVSG
QSSFGTSPAADNIEKEALPRYEPIPFSASMNESTPTGITDHIAQGRNFS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC006937
ORF Size 567 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006937, AAH06937
RefSeq Size 3281 bp
RefSeq ORF 569 bp
Locus ID 68095
Cytogenetics 5 C3.2
Summary Maintains stem cell potency (PubMed:23972987). Increases STAT3 phosphorylation and controls ERK phosphorylation (PubMed:23972987). May act as a scaffold, increasing STAT3 recruitment onto endosomes (PubMed:23972987).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC006937" in other vectors (1)
SKU Description Size Price
MC200272 Ociad1 (untagged) - Mouse OCIA domain containing 1 (cDNA clone MGC:6810 IMAGE:2648369), (10ug) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.