9430016H08Rik (BC024945) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201651
9430016H08Rik (tGFP-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 9430016H08Rik
Synonyms AA409999; C78988
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201651 representing BC024945
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATTCTGGGATTTTCCAATCCCATCAACTGGGTCCGGACTCGAATTTACGCCTTCCTTATTTGGG
CCTATTTCGACAAAGAGTTCAGTATCGCTGAGTTCTCTGAAGGGGCGAAGCAGGCTTTTGCTTATGTATC
CAAGTTGCTGTCACAGTGTAAATTTGATCTACTGGAAGAACTTGTAGCCAAAGAGGTGCTACAGATATTA
AAAGAAAAAGTTACTTCATTGTCTGATAACCATAAGAATGCACTTGCTGCAGACATAGATGACATTGTCT
ACACATCAACAGGCGATATCTCCATTTACTATGATGAGAAAGGAAGGAAGTTTGTTAACATCCTGATGTG
CTTTTGGTATCTGACTAGTGCCAACATCCCCAGTGAGTCTTTAAGTGGAGCCAATGTGTTCCAGGTGAAG
CTGGGGGATCAGAGTGTGGAAACTAAACAGCTGCTCAGTGCAAGCTATGAATTTCAGAGGGAATTCACAC
AAGGAGTAAAGCCTGATTGGACGATTGCGCGGATTGAACACTCCAAGTTACTAGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201651 representing BC024945
Red=Cloning site Green=Tags(s)

MIILGFSNPINWVRTRIYAFLIWAYFDKEFSIAEFSEGAKQAFAYVSKLLSQCKFDLLEELVAKEVLQIL
KEKVTSLSDNHKNALAADIDDIVYTSTGDISIYYDEKGRKFVNILMCFWYLTSANIPSESLSGANVFQVK
LGDQSVETKQLLSASYEFQREFTQGVKPDWTIARIEHSKLLE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC024945
ORF Size 546 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024945, AAH24945
RefSeq Size 839 bp
RefSeq ORF 548 bp
Locus ID 68115
Cytogenetics 1 C1.3
Summary Promotes sorting of SMDT1/EMRE in mitochondria by ensuring its maturation. Interacts with the transit peptide region of SMDT1/EMRE precursor protein in the mitochondrial matrix, leading to protect it against protein degradation by YME1L1, thereby ensuring SMDT1/EMRE maturation by the mitochondrial processing peptidase (PMPCA and PMPCB).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC024945" in other vectors (4)
SKU Description Size Price
MC206919 9430016H08Rik (untagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472), (10ug) 10 ug
$330.00
MR201651 9430016H08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) 10 ug
$289.00 $300.00
MR201651L3 Lenti ORF clone of 9430016H08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) 10 ug
$600.00
MR201651L4 Lenti ORF clone of 9430016H08Rik (mGFP-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) 10 ug
$600.00

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