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Fam89b (NM_023166) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201546
Fam89b (tGFP-tagged) - Mouse mammary tumor virus receptor 2 (Mtvr2), transcript variant 2
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Fam89b or NM_023166"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fam89b
Synonyms 1110021A21Rik; MMTV; Mtvr2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201546 representing NM_023166
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGGCTGCCCGCGACTGAGGCGCCGGGCGGCGCGGGCTGCGCTCTGGCCGGGCTCCCGCCGCTAC
CGCGCGGCCTCAGCGGCCTTCTTAACGCTAGTGGGGGCTCGTGGAGGGAGCTGGAGCGTGTCTACAGCCA
ACGGAGCCGCATCCACGACGAACTGAGTCGTGCCGCCCGCGCCCCGGATGGTCCTCGCCATGCCGCTGGA
TCTGCTAACTCAGGTTCTGCAGCGGGCCCGCGCCGCCCGGTTAACCTCGACTCTGCACTAGCCGCGCTGC
GCAAGGAGATGCTGTGGGGCCTGTACGAGTCGATCCAGGACTACAAGCATCTGTGCCAAGACCTGAGCTT
GTGCCAGGACCTGTCATCCTCTCTGCACTCGGACAGCTCCTACCCACCTGATGCCGGCCTGTCTGATGAC
GAGGAGCCTCCTGATGCCAGCCTGCCCCCAGACCCTCCACCCCTCACCGTACCCCAGACTCACAATGCCC
GTGACCAGTGGTTGCAGGATGCTTTCCAAATCAGCCTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201546 representing NM_023166
Red=Cloning site Green=Tags(s)

MNGLPATEAPGGAGCALAGLPPLPRGLSGLLNASGGSWRELERVYSQRSRIHDELSRAARAPDGPRHAAG
SANSGSAAGPRRPVNLDSALAALRKEMLWGLYESIQDYKHLCQDLSLCQDLSSSLHSDSSYPPDAGLSDD
EEPPDASLPPDPPPLTVPQTHNARDQWLQDAFQISL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023166
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023166.2
RefSeq Size 1142 bp
RefSeq ORF 531 bp
Locus ID 17826
Cytogenetics 19 4.34 cM
Summary Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta (PubMed:12646588). Acts as an adapter that mediates the specific recognition of LIMK1 by CDC42BPA and CDC42BPB in the lamellipodia. LRAP25-mediated CDC42BPA/CDC42BPB targeting to LIMK1 and the lamellipodium results in LIMK1 activation and the subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023166" in other vectors (4)
SKU Description Size Price
MC205091 Fam89b (untagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2, (10ug) 10 ug
$300.00
MR201546 Fam89b (Myc-DDK-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2 10 ug
$289.00 $300.00
MR201546L3 Lenti ORF clone of Fam89b (Myc-DDK-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2 10 ug
$600.00
MR201546L4 Lenti ORF clone of Fam89b (mGFP-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2 10 ug
$600.00

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