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Fopnl (NM_025345) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201498
Fopnl (tGFP-tagged) - Mouse RIKEN cDNA 0610037P05 gene (0610037P05Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Fopnl or NM_025345"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fopnl
Synonyms 0610037P05Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201498 representing NM_025345
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTGTTACGGAACTGAAAGCTGTTTTAAAGGACACCTTGGAAAAAAGGGGAGTGTTAGGACATT
TAAAAGCAAGGATCCGTGCTGAAGTTTTCAATGCCCTGGATGATGATCGTGAACCCCGACCGTCTTTGTC
TCATGAGAACCTTCTAATTAATGAATTAATTAGGGAGTATTTGGAATTCAATAAATATAAATACACAGCC
TCTGTCCTCATAGCAGAATCTGGTCAACCTGTAGTTCCATTGGACAGACAGTTTCTCATCCGTGAACTAA
ATGCCTTTGAAGAATCAAAGGATAACTCAATTCCTCTATTGTATGGAATTTTAGCCCATTTCTTGCGTGG
TCCTCCAGATGGAGCCCAGAATGTGCTTCTGACAGAGTCAACCCTGCACCCGGCAACCAAGCATCTCAGC
TGGAAGCCCAGCAGAAGACCTGACGATGACCACGTGAGGAAGGACACAGGGCCAAGAACCACAACTGAAG
AGCTTCCTGCTGCTGCACAAGCAGTCAGCAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201498 representing NM_025345
Red=Cloning site Green=Tags(s)

MATVTELKAVLKDTLEKRGVLGHLKARIRAEVFNALDDDREPRPSLSHENLLINELIREYLEFNKYKYTA
SVLIAESGQPVVPLDRQFLIRELNAFEESKDNSIPLLYGILAHFLRGPPDGAQNVLLTESTLHPATKHLS
WKPSRRPDDDHVRKDTGPRTTTEELPAAAQAVSR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025345
ORF Size 522 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025345.2, NP_079621.1
RefSeq Size 1438 bp
RefSeq ORF 525 bp
Locus ID 66086
UniProt ID Q9CZS3
Cytogenetics 16 A1
Summary Involved in the biogenesis of cilia (By similarity). Required for the recruitement of PLK1 to centrosomes and S phase progression (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025345" in other vectors (4)
SKU Description Size Price
MC200958 Fopnl (untagged) - Mouse RIKEN cDNA 0610037P05 gene (0610037P05Rik), (10ug) 10 ug
$300.00
MR201498 Fopnl (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610037P05 gene (0610037P05Rik) 10 ug
$289.00 $300.00
MR201498L3 Lenti ORF clone of Fopnl (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610037P05 gene (0610037P05Rik) 10 ug
$600.00
MR201498L4 Lenti ORF clone of Fopnl (mGFP-tagged) - Mouse RIKEN cDNA 0610037P05 gene (0610037P05Rik) 10 ug
$600.00

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