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Nenf (BC048464) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201443
Nenf (tGFP-tagged) - Mouse neuron derived neurotrophic factor (cDNA clone MGC:58200 IMAGE:6596336)
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nenf
Synonyms Spuf, SCIRP10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201443 representing BC048464
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGCCCCGCGCCCTGGTGGCGGCTGCGGCTGCTGGCGGCGCTCGTCCTGGCGCTGGCTCTGGTCC
CAGTGCCCTCAGCCTGGGCTGGGCAGACGCCGCGCCCCGCAGAGCGCGGGCCCCCGGTGCGGCTCTTCAC
CGAGGAGGAGCTGGCCCGCTACGGCGGCGAGGAGGAGGATCAGCCCATCTACTTGGCAGTGAAGGGAGTG
GTGTTCGATGTCACCTCTGGGAAGGAGTTTTATGGACGAGGAGCCCCCTACAATGCCTTGGCCGGGAAGG
ACTCCAGCAGAGGTGTGGCCAAGATGTCACTGGATCCAGCAGACCTCACTCACGACACTACTGGTCTCAC
GGCCAAGGAGCTGGAGGCCCTCGATGACGTCTTCAGCAAGGTGTACAAAGCCAAGTACCCCATTGTTGGC
TACACAGCCCGAAGGATCCTCAACGAGGATGGCAGCCCCAACCTGGACTTCAAGCCTGAAGACCAGCCCC
ATTTTGACATAAAGGACGAATTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC048464
ORF Size 513 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC048464, AAH48464
RefSeq Size 681 bp
RefSeq ORF 515 bp
Locus ID 66208
Cytogenetics 1 H6
Summary Acts as a neurotrophic factor in postnatal mature neurons, enhancing neuronal survival (PubMed:15605373). Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte (PubMed:16547973). Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways (PubMed:15605373, PubMed:16547973). Neurotrophic activity is enhanced by binding to heme (PubMed:18056703). Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (PubMed:23576617).UniProtKB/Swiss-Prot Function
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