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Golga7b (NM_027694) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201370
Golga7b (tGFP-tagged) - Mouse RIKEN cDNA 4933417O08 gene (4933417O08Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Golga7b
Synonyms 4933417O08Rik; AI839934
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201370 representing NM_027694
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGAGGTCCACAATCTTCAGGAGCTCAGGCGAAGCGCTTCGCTGGCCACCAAGGTCTTTATCC
AGAGAGACTACAGTGACGGCACCATCTGTCAGTTCCAGACCAAATTCCCGCCAGAGCTGGATAGCCGGAT
TGAGCGGCAGCTCTTTGAGGAGACGGTGAAGACCCTCAATGGTTTTTATGCGGAGGCTGAGAAGATCGGG
GGCAGCTCCTATCTGGAGGGCTGCCTGGCCTGCGCCACGGCCTACTTCATCTTCCTCTGCATGGAGACCC
ACTATGAGAAGGTTCTCAAGAAGATTTCCCGTTACATCCAGGAGCAGAATGAGAAGGTCTTTGCTCCCCG
AGGGCTCCTGCTCACGGATCCGGTGGAACGCGGAATGAGGGTTATCGAGATCTCCATCTATGAGGACCGG
TGCAGCAGTGGTAGTTCCAGTAGCGGCAGCAGCAGCGGCAGCGGCAGCAGCAGCGCTGGCGGGGGAGGGG
CGGGGGCCCGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201370 representing NM_027694
Red=Cloning site Green=Tags(s)

MATEVHNLQELRRSASLATKVFIQRDYSDGTICQFQTKFPPELDSRIERQLFEETVKTLNGFYAEAEKIG
GSSYLEGCLACATAYFIFLCMETHYEKVLKKISRYIQEQNEKVFAPRGLLLTDPVERGMRVIEISIYEDR
CSSGSSSSGSSSGSGSSSAGGGGAGAR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027694
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027694.2, NP_081970.1
RefSeq Size 2820 bp
RefSeq ORF 504 bp
Locus ID 71146
UniProt ID Q9D428
Cytogenetics 19 C3
Summary May be involved in protein transport from Golgi to cell surface.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027694" in other vectors (4)
SKU Description Size Price
MC211147 Golga7b (untagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 2, (10ug) 10 ug
$330.00
MR201370 Golga7b (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 2 10 ug
$289.00 $300.00
MR201370L3 Lenti ORF clone of Golga7b (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 2 10 ug
$600.00
MR201370L4 Lenti ORF clone of Golga7b (mGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 2 10 ug
$600.00

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