Cfl1 (NM_007687) Mouse Tagged ORF Clone

CAT#: MG201348

  • TrueORF®

Cfl1 (tGFP-tagged) - Mouse cofilin 1, non-muscle (Cfl1)


  "NM_007687" in other vectors (5)

Reconstitution Protocol

USD 650.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Cfl1
Synonyms AA959946; Cof
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG201348 representing NM_007687
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTGGTGTGGCTGTCTCTGATGGTGTCATCAAGGTGTTCAATGACATGAAGGTTCGCAAGTCTT
CAACACCAGAAGAAGTGAAGAAACGCAAGAAGGCGGTGCTCTTTTGCCTGAGTGAGGACAAGAAGAACAT
CATCCTGGAGGAGGGCAAGGAGATCCTGGTAGGAGATGTGGGGCAGACTGTGGACGACCCCTACACCACT
TTTGTCAAGATGCTGCCAGACAAGGACTGCCGCTATGCACTCTATGATGCAACCTATGAGACCAAGGAGA
GCAAGAAGGAGGACCTGGTGTTCATCTTCTGGGCCCCCGAGAATGCACCCCTCAAGAGCAAAATGATCTA
TGCCAGCTCCAAGGATGCCATCAAGAAGAAGCTGACAGGAATCAAGCATGAATTACAAGCTAACTGCTAC
GAGGAGGTCAAGGACCGCTGCACCCTGGCAGAGAAACTAGGTGGCAGCGCCGTCATTTCCCTGGAGGGCA
AGCCTTTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG201348 representing NM_007687
Red=Cloning site Green=Tags(s)

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYTT
FVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPENAPLKSKMIYASSKDAIKKKLTGIKHELQANCY
EEVKDRCTLAEKLGGSAVISLEGKPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007687
ORF Size 498 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007687.5, NP_031713.1
RefSeq Size 1196 bp
RefSeq ORF 501 bp
Locus ID 12631
UniProt ID P18760
Cytogenetics 19 A
Gene Summary Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.