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Rpl12 (BC081469) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201324
Rpl12 (tGFP-tagged) - Mouse ribosomal protein L12 (cDNA clone MGC:102182 IMAGE:6824411)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpl12
Synonyms E430018F03
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201324 representing BC081469
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCCAAGTTCGACCCCAACGAGGTCAAAGTCGTGTACCTGAGGTGCACCGGAGGCGAGGTCGGCG
CCACATCCGCCTTGGCCCCGAAGATCGGTCCTCTGGGTCTGTCTCCGAAGAAAGTTGGCGATGACATTGC
CAAGGCTACCGGTGACTGGAAGGGTCTCAGAATTACAGTGAAACTGACCATCCAGAACAGACAGGCCCAG
ATCGAGGTGGTGCCTTCTGCCTCAGCCCTGATCATCAAAGCCCTCAAGGAGCCACCGAGAGACAGGAAGA
AGCAGAAGAACATTAAACACAGTGGGAACATTACTTTTGATGAGATTGTCAACATTGCCCGGCAGATGAG
ACACCGGTCTTTGGCTAGAGAACTTTCTGGAACTATCAAGGAGATCCTGGGTACTGCACAGTCTGTGGGC
TGCAATGTGGATGGCCGCCACCCTCATGACATCATAGATGACATCAACAGTGGTGCAGTGGAGTGCCCAG
CTAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201324 representing BC081469
Red=Cloning site Green=Tags(s)

MPPKFDPNEVKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQ
IEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVG
CNVDGRHPHDIIDDINSGAVECPAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC081469
ORF Size 495 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC081469, AAH81469
RefSeq Size 640 bp
RefSeq ORF 497 bp
Locus ID 269261
Cytogenetics 2 B
Summary Binds directly to 26S ribosomal RNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC081469" in other vectors (4)
SKU Description Size Price
MC206995 Rpl12 (untagged) - Mouse ribosomal protein L12 (cDNA clone MGC:102182 IMAGE:6824411), (10ug) 10 ug
$165.00
MR201324 Rpl12 (Myc-DDK-tagged) - Mouse ribosomal protein L12 (cDNA clone MGC:102182 IMAGE:6824411) 10 ug
$289.00
MR201324L3 Lenti ORF clone of Rpl12 (Myc-DDK-tagged) - Mouse ribosomal protein L12 (cDNA clone MGC:102182 IMAGE:6824411) 10 ug
$450.00
MR201324L4 Lenti ORF clone of Rpl12 (mGFP-tagged) - Mouse ribosomal protein L12 (cDNA clone MGC:102182 IMAGE:6824411) 10 ug
$450.00

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