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Tmem138 (NM_028411) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201271
Tmem138 (tGFP-tagged) - Mouse transmembrane protein 138 (Tmem138)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem138
Synonyms 1700113I01Rik; 2900055D14Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201271 representing NM_028411
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCAGACCGGTAACTACAGCCTGGTGCTCTCCCTGCAGTTCCTGTTGCTGTCCTATGACCTGTTCG
TCAACTCCTTCTCAGAGCTACTCCGAATGGCTCCTGTTATCCAGCTTGTGCTGTTCATCATCCAGGATAT
TGCAATCCTCTTCAACATCATCATAATTTTCCTCATGTTCTTCAACACCTTCGTCTTCCAGGCTGGCCTG
GTCAACCTCCTTTTCCATAAGTTCAAAGGGACCATCATTCTGACATCTGTGTACCTTGCCCTCAGCATCT
CCCTACATGTCTGGGTCATGAACGTGCGATGGAAAAACTCCAGCAGCTTCAGCTGGACAAACGGCCTGCA
AACACTGTTTGTATTCCAGAGACTAGCCGCAGTGCTCTACTGCTATTTCTACAAAAGGACGGCCGTGAGA
CTGGGTGACCCCCGCTTTTACCAGGACTCACTGTGGCTTCGCAAGGAGTTCATGCAAGTCCGAAGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201271 representing NM_028411
Red=Cloning site Green=Tags(s)

MLQTGNYSLVLSLQFLLLSYDLFVNSFSELLRMAPVIQLVLFIIQDIAILFNIIIIFLMFFNTFVFQAGL
VNLLFHKFKGTIILTSVYLALSISLHVWVMNVRWKNSSSFSWTNGLQTLFVFQRLAAVLYCYFYKRTAVR
LGDPRFYQDSLWLRKEFMQVRR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028411
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028411.4, NP_082687.1
RefSeq Size 918 bp
RefSeq ORF 489 bp
Locus ID 72982
UniProt ID Q9D6G5
Cytogenetics 19 A
Summary Required for ciliogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028411" in other vectors (4)
SKU Description Size Price
MC211374 Tmem138 (untagged) - Mouse transmembrane protein 138 (Tmem138), (10ug) 10 ug
$165.00
MR201271 Tmem138 (Myc-DDK-tagged) - Mouse transmembrane protein 138 (Tmem138) 10 ug
$289.00
MR201271L3 Lenti ORF clone of Tmem138 (Myc-DDK-tagged) - Mouse transmembrane protein 138 (Tmem138) 10 ug
$450.00
MR201271L4 Lenti ORF clone of Tmem138 (mGFP-tagged) - Mouse transmembrane protein 138 (Tmem138) 10 ug
$450.00

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