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Tnnc2 (NM_009394) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201248
Tnnc2 (tGFP-tagged) - Mouse troponin C2, fast (Tnnc2)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tnnc2
Synonyms Tncs
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201248 representing NM_009394
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGACCAACAGGCTGAGGCCAGGTCCTACCTCAGCGAGGAGATGATCGCTGAGTTCAAGGCTGCCT
TTGACATGTTCGATGCTGATGGCGGTGGGGACATCAGCGTTAAAGAGTTGGGCACCGTGATGAGGATGCT
AGGGCAGACACCCACCAAAGAGGAATTGGATGCCATCATCGAGGAGGTGGACGAGGATGGCAGCGGTACT
ATCGACTTTGAAGAGTTCTTGGTCATGATGGTGCGCCAGATGAAAGAGGATGCGAAGGGGAAGAGCGAAG
AGGAACTGGCTGAGTGCTTCCGCATCTTTGACAGGAACGCAGACGGCTACATTGATGCTGAGGAGCTAGC
TGAGATTTTCCGGGCTTCTGGGGAGCATGTGACAGAAGAGGAGATCGAATCCCTGATGAAGGATGGTGAT
AAAAACAACGACGGCCGCATTGACTTTGATGAGTTTCTGAAGATGATGGAGGGCGTTCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201248 representing NM_009394
Red=Cloning site Green=Tags(s)

MTDQQAEARSYLSEEMIAEFKAAFDMFDADGGGDISVKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGT
IDFEEFLVMMVRQMKEDAKGKSEEELAECFRIFDRNADGYIDAEELAEIFRASGEHVTEEEIESLMKDGD
KNNDGRIDFDEFLKMMEGVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009394
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009394.2, NP_033420.1
RefSeq Size 705 bp
RefSeq ORF 483 bp
Locus ID 21925
UniProt ID P20801
Cytogenetics 2 H3
Summary Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009394" in other vectors (4)
SKU Description Size Price
MC201847 Tnnc2 (untagged) - Mouse troponin C2, fast (Tnnc2), (10ug) 10 ug
$150.00
MR201248 Tnnc2 (Myc-DDK-tagged) - Mouse troponin C2, fast (Tnnc2) 10 ug
$289.00
MR201248L3 Lenti ORF clone of Tnnc2 (Myc-DDK-tagged) - Mouse troponin C2, fast (Tnnc2) 10 ug
$450.00
MR201248L4 Lenti ORF clone of Tnnc2 (mGFP-tagged) - Mouse troponin C2, fast (Tnnc2) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.