Rbm38 (BC006687) Mouse Tagged ORF Clone

CAT#: MG201195

Rbm38 (tGFP-tagged) - Mouse RNA binding motif protein 38 (cDNA clone MGC:6122 IMAGE:3582604)

Product Images

Circular map for MG201195

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rbm38
• Synonyms: Rnpc1; Seb4; Seb4l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201195 representing BC006687
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATCGGGCAGCGGCTGATAGGGCTTGCAAAGACCCTAACCCTATCATCGATGGTCGCAAGGCCA
  ATGTGAACCTGGCCTACCTGGGTGCCAAGCCTAGGAGCTTACAGACGGGCTTTGCTGTTGGTGTGCAGCA
  ACTACACCCCACCTTGATCCAGCGCACCTACGGGCTGACTCCTCACTACATCTACCCACCAGCCATTGTG
  CAGCCCAGCGTGGTGATCCCTGCCACCCCTGTCCCGTCACTGTCCTCGCCCTACCTTGAGTATACACCAG
  CCAGCCCAGCCTACGCCCAGTACCCGCCAGCCACTTACGACCAGTACCCATATGCTGCCTCACCTGCTGC
  AGCCACCAGCTTCGTGGGCTACGGCTACCCTGCTGCCGTGCCCCAAGCCTTGTCTGCTGCTGCACCTGCA
  GGCACCACCTTCGTGCAGTACCAGGCACCTCAGCTACAGCCTGACAGGATGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201195 representing BC006687
  Red=Cloning site Green=Tags(s)
  
  MADRAAADRACKDPNPIIDGRKANVNLAYLGAKPRSLQTGFAVGVQQLHPTLIQRTYGLTPHYIYPPAIV
  QPSVVIPATPVPSLSSPYLEYTPASPAYAQYPPATYDQYPYAASPAAATSFVGYGYPAAVPQALSAAAPA
  GTTFVQYQAPQLQPDRMQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006687
• ORF Size: 476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006687
• RefSeq Size: 2308 bp
• RefSeq ORF: 476 bp
• Locus ID: 56190
• Cytogenetics: 2 H3
• Gene Summary: RNA-binding protein that specifically bind the 3' UTR of CDKN1A transcripts, leading to maintain the stability of CDKN1A transcripts, thereby acting as a mediator of the p53/TP53 family to regulate CDKN1A. CDKN1A is a cyclin-dependent kinase inhibitor transcriptionally regulated by the p53/TP53 family to induce cell cycle arrest. Has the ability to induce cell cycle arrest in G1 and maintain the stability of CDKN1A transcripts induced by p53/TP53. Also acts as a mRNA splicing factor. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 (By similarity). Plays a role in myogenic differentiation.[UniProtKB/Swiss-Prot Function]