Cplx3 (NM_146223) Mouse Tagged ORF Clone

CAT#: MG201191

Cplx3 (tGFP-tagged) - Mouse complexin 3 (Cplx3)

Product Images

Circular map for MG201191

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cplx3
• Synonyms: BC016632; CpxIII; ERGIC-53L; ERGL; Lamn1l; Lman1l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201191 representing NM_146223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCATGGTGAAGTCCATGGTGGGTGGCCAACTGAAGAACCTCACAGGGAGCCTGGGGGGCGGCG
  AGGACAAGGGGGACGGAGACAAGTCTGCAGCCGAAGCACAGGGCATGAGCCGAGAAGAGTACGAGGAGTA
  TCAGAAGCAACTGGTGGAGGAGAAGATGGAGCGAGATGCACAGTTCACACAGAGGAAGGCAGAGCGGGCC
  ACACTGCGAAGTCACTTCAGAGACAAATACCGTCTGCCCAAGAACGAAACAGATGAAAGCCAGATCCAGC
  TGGCAGGCGGAGATGTGGAGCTGCCTCGGGAGCTAGCCAAGATGATCGAGGAGGATACGGAGGAAGAGGA
  GGATAAGGCCTCTGTGCTCGGGCAGCTGGCCAGCCTCCCTGGCCTAGACCTCAGCTCACTCAAGGACAAA
  GCCCAGACTACACTAGGGGACCTCAAGCAATCAGCTGAGAAGTGCCATATCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201191 representing NM_146223
  Red=Cloning site Green=Tags(s)
  
  MAFMVKSMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERA
  TLRSHFRDKYRLPKNETDESQIQLAGGDVELPRELAKMIEEDTEEEEDKASVLGQLASLPGLDLSSLKDK
  AQTTLGDLKQSAEKCHIM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146223
• ORF Size: 474 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146223.3
• RefSeq Size: 2876 bp
• RefSeq ORF: 477 bp
• Locus ID: 235415
• UniProt ID: Q8R1B5
• Cytogenetics: 9 B
• Gene Summary: Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (PubMed:19386896). Required for the maintenance of synaptic ultrastructure in the adult retina (PubMed:19386896). Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (PubMed:15911881, PubMed:19386896, PubMed:27335398). Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+) vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+) vesicle release (PubMed:22694764, PubMed:27335398).[UniProtKB/Swiss-Prot Function]