Diablo (BC024780) Mouse Tagged ORF Clone

CAT#: MG201181

Diablo (tGFP-tagged) - Mouse diablo homolog (Drosophila) (cDNA clone MGC:35815 IMAGE:5365266)

Product Images

Circular map for MG201181

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Diablo
• Synonyms: 0610041G12Rik; 1700006L01Rik; AU040403; Smac
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201181 representing BC024780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCTGAGAAGTTGGGTGACTCGGAGCGTCTGCTCTCTGTTCAGGTACAGACAGCGTTTTCCTG
  TCTTGGCTAACTCTAAGAAACGCTGTTTCTCAGAATTGATAAAACCATGGCACAAAACTGTGTTGACTGG
  ATTTGGCATGACACTGTGTGCGGTTCCTATTGCTCAGAAATCGGAGCCTCAGTCTCTCAGTAACGAAGCA
  TTGATGAGGAGGGCTGTGTCTTTGGTAACAGATAGCACCTCTACCTTTCTGTCTCAAACCACCTACGCGC
  TGATTGAAGCAATCACCGAGTATACTAAGGCTGTTTACACGTTAGTGTCTCTGTACCGACAATATACAAG
  TTTACTTGGGAAGATGAATTCCCAGGAGGAAGATGAGGTGTGGCAGGTAATTATAGGAGCCAGAGTTGAG
  GTGAGTGAACCTGTTAGTCATTTCCATATTAGTGGGCTTCATTATGGCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201181 representing BC024780
  Red=Cloning site Green=Tags(s)
  
  MAALRSWVTRSVCSLFRYRQRFPVLANSKKRCFSELIKPWHKTVLTGFGMTLCAVPIAQKSEPQSLSNEA
  LMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLVSLYRQYTSLLGKMNSQEEDEVWQVIIGARVE
  VSEPVSHFHISGLHYGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024780
• ORF Size: 471 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024780.1
• RefSeq Size: 2585 bp
• RefSeq ORF: 473 bp
• Locus ID: 66593
• Cytogenetics: 5 F
• Gene Summary: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity).[UniProtKB/Swiss-Prot Function]