Ap1s3 (BC098237) Mouse Tagged ORF Clone

CAT#: MG201140

Ap1s3 (tGFP-tagged) - Mouse adaptor-related protein complex AP-1, sigma 3 (cDNA clone MGC:106818 IMAGE:3825593)

Product Images

Circular map for MG201140

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap1s3
• Synonyms: [s]3A, Jr2, 1190009B22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201140 representing BC098237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACATTTCATCTTGCTCTTCAGTCGACAAGGGAAGCTGCGGCTGCAGAAATGGTACACCACACTCC
  CTGACAAGGAGAGGAAGAAGATCACCCGCGACATCATCCAGACCGTCCTCTCTCGTGGGCACAGGACCAG
  CAGCTTCATTGACTGGAAGGAGCTGAAACTTGTTTATAAAAGGTATGCTAGTTTATATTTTTGCTGTGCA
  ATAGAAAATCAAGACAATGAGCTCTTGACACTAGAGATTGTTCATCGTTATGTGGAGTTGCTGGATAAAT
  ACTTTGGAAATGTCTGTGAGCTGGATATTATCTTTAATTTTGAAAAGGCATATTTTATCCTTGATGAGTT
  TATAATAGGTGGAGAAATTCAGGAAACATCCAAGAAAACAGCAGTCAAGGCCATTGAAGACTCTGATATG
  TTACAAGAGACAATGGAAGAATACATGAACAAGCCCACGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201140 representing BC098237
  Red=Cloning site Green=Tags(s)
  
  MIHFILLFSRQGKLRLQKWYTTLPDKERKKITRDIIQTVLSRGHRTSSFIDWKELKLVYKRYASLYFCCA
  IENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIGGEIQETSKKTAVKAIEDSDM
  LQETMEEYMNKPTF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC098237
• ORF Size: 462 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC098237.1
• RefSeq Size: 1298 bp
• RefSeq ORF: 464 bp
• Locus ID: 252903
• Cytogenetics: 1 C4
• Gene Summary: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules (By similarity). Involved in TLR3 trafficking (By similarity).[UniProtKB/Swiss-Prot Function]