Ccdc184 (NM_177716) Mouse Tagged ORF Clone

CAT#: MG201100

AI836003 (tGFP-tagged) - Mouse expressed sequence AI836003 (AI836003)

Product Images

Circular map for MG201100

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc184
• Synonyms: 5630400A08; AI836003
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201100 representing NM_177716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAACTGATGGAGCACCTGAAGGCCCAGCTGCAGGCCCTGTTTGAGGACGTGAGGGCCATGCGAG
  GGGCACTGGACGAGCAGGCCTCGCACATCCAGGTTCTGTCGGACGACGTGTGCGCCAACCAGCGGGCTAT
  AGTCTCCATGTGCCAGATTATGACCACTGCGCCCCGCCAGGGAGGCTTGGGCGTGGCCGGCGGCAAGGGG
  AGCTTCCCAAGTGTTCCCCATGAACCAGAGACACCTTCGCCTGGGATCGAGGACAGCGGCTTGCTGGGTC
  GTGATCCTGATGAAGACGAGGACGAAGAGGAGAAAGAGACACCTAACCCCGCCACACCCACCAGTCACTG
  CGAGCTCTCTGAGAGCCCCGGTCTCCTTGGGGAGGATGGGCCACTTGTGGAGCCCCCCGACCTTCCTGAC
  ATTACCCTGCTGCAGCTGGAGGGCGAGGCCTCTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201100 representing NM_177716
  Red=Cloning site Green=Tags(s)
  
  MKELMEHLKAQLQALFEDVRAMRGALDEQASHIQVLSDDVCANQRAIVSMCQIMTTAPRQGGLGVAGGKG
  SFPSVPHEPETPSPGIEDSGLLGRDPDEDEDEEEKETPNPATPTSHCELSESPGLLGEDGPLVEPPDLPD
  ITLLQLEGEASL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177716
• ORF Size: 456 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177716.1, NP_808384.1
• RefSeq Size: 2471 bp
• RefSeq ORF: 576 bp
• Locus ID: 239650
• UniProt ID: Q8BMK5
• Cytogenetics: 15 F1