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Wtap (NM_175394) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201073
Wtap (tGFP-tagged) - Mouse Wilms' tumour 1-associating protein (Wtap)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Wtap or NM_175394"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wtap
Synonyms 2810408K05Rik; 9430038B09Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201073 representing NM_175394
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAACGAAGAACCTCTTCCTAAAAAGGTCCGACTGAGTGAAACAGACTTCAAAGTTATGGCACGGG
ATGAGTTAATTCTAAGATGGAAACAATATGAAGCATATGTTCAAGCTTTGGAGGGAAAGTACACAGATCT
TAATTCAAACGATGTGACTGGTTTAAGGGAATCTGAAGAAAAACTAAAGCAGCAACAGCAGGAGTCTGCA
CGCAGGGAGAACATTCTTGTCATGCGGCTAGCAACCAAGGAGCAGGAGATGCAAGAGTGCACTACTCAAA
TCCAGTACCTCAAGCAAGTTCAGCAGCCGAGTGTGGCCCAACTGAGATCAACAATGGTAGACCCAGCGAT
CAACTTGTTTTTCCTAAAAATGAAAGGTGAACTGGAACAGACTAAAGACAAACTGGAACAAGCCCAAAAT
GAACTGAGTGCCTGGAAGTTTACGCCTGATAGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201073 representing NM_175394
Red=Cloning site Green=Tags(s)

MTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESA
RRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQN
ELSAWKFTPDR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175394
ORF Size 453 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175394.1, NP_780603.1
RefSeq Size 3309 bp
RefSeq ORF 456 bp
Locus ID 60532
UniProt ID Q9ER69
Cytogenetics 17 A1
Summary Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3' UTR (PubMed:29547716). Required for accumulation of METTL3 and METTL14 to nuclear speckle (By similarity). Acts as a mRNA splicing regulator (By similarity). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (By similarity). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175394" in other vectors (4)
SKU Description Size Price
MC207546 Wtap (untagged) - Mouse Wilms' tumour 1-associating protein (Wtap), transcript variant 2, (10ug) 10 ug
$165.00
MR201073 Wtap (Myc-DDK-tagged) - Mouse Wilms' tumour 1-associating protein (Wtap), transcript variant 2 10 ug
$289.00
MR201073L3 Lenti ORF clone of Wtap (Myc-DDK-tagged) - Mouse Wilms' tumour 1-associating protein (Wtap), transcript variant 2 10 ug
$450.00
MR201073L4 Lenti ORF clone of Wtap (mGFP-tagged) - Mouse Wilms' tumour 1-associating protein (Wtap), transcript variant 2 10 ug
$450.00

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