Mtvr2 (BC013894) Mouse Tagged ORF Clone

CAT#: MG201035

Mtvr2 (tGFP-tagged) - Mouse mammary tumor virus receptor 2 (cDNA clone MGC:13743 IMAGE:4217979)

Product Images

Circular map for MG201035

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mtvr2
• Synonyms: 1110021A21Rik; MMTV; Mtvr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201035 representing BC013894
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGGCTGCCCGCGACTGAGGCGCCGGGCGGCGCGGGCTGCGCTCTGGCCGGGCTCCCGCCGCTAC
  CGCGCGGCCTCAGCGGCCTTCTTAACGCTAGTGGGGGCTCGTGGAGGGAGCTGGAGCGTGTCTACAGCCA
  ACGGAGCCGCATCCACGACGAACTGAGTCGTGCCGCCCGCGCCCCGGATGGTCCTCGCCATGCCGCTGGA
  TCTGCTAACTCAGGTTCTGCAGCGGGCCCGCGCCGCCCGGTTAACCTCGACTCTGCACTAGCCGCGCTGC
  GCAAGGAGATGTTGTCTGTAGGTGGGGCTGCGACAGCTGGATATGTCCCTGTTGTGCCAGCTGTGGGGCC
  TGTACGAGTCGATCCAGGACTACAAGCATCTGTGCCAAGACCTGAGCTTGTGCCAGGACCTGTCATCCTC
  TCTGCACTCGGACAGCTCCTACCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201035 representing BC013894
  Red=Cloning site Green=Tags(s)
  
  MNGLPATEAPGGAGCALAGLPPLPRGLSGLLNASGGSWRELERVYSQRSRIHDELSRAARAPDGPRHAAG
  SANSGSAAGPRRPVNLDSALAALRKEMLSVGGAATAGYVPVVPAVGPVRVDPGLQASVPRPELVPGPVIL
  SALGQLLPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013894
• ORF Size: 447 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013894.1
• RefSeq Size: 1218 bp
• RefSeq ORF: 449 bp
• Locus ID: 17826
• Cytogenetics: 19 4.34 cM
• Gene Summary: Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta (PubMed:12646588). Acts as an adapter that mediates the specific recognition of LIMK1 by CDC42BPA and CDC42BPB in the lamellipodia. LRAP25-mediated CDC42BPA/CDC42BPB targeting to LIMK1 and the lamellipodium results in LIMK1 activation and the subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function]