Cst10 (BC048364) Mouse Tagged ORF Clone

CAT#: MG201025

Cst10 (tGFP-tagged) - Mouse cystatin 10 (chondrocytes) (cDNA clone MGC:58889 IMAGE:4920219)

Product Images

Circular map for MG201025

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cst10
• Synonyms: DD72
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201025 representing BC048364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGTTTGCTGAGCCCCTCGATGCCTGTGTTGGCTGCTGTGGCCTTGACACTGACCCTGGCTGTGA
  TCCCTGAAGCCAGTACTAATGCTGAAGCCAAGCAGGTGGTATTAGGTGGAGTGGAGCCAGCTGACCCTAA
  GGATAAGGAGGTGCAGAAAGTGGTAAAGTTTGCTGTTAGAACCTACAATGATATGGACAATGACCTGTAT
  CTTAGCAAGCCAATACGACTGATGAGCGCCAGCCAGCAGGTTGTGGCTGGGAAGAACTACTACTTGAAAA
  TTGAGCTAGGCCGAACCACATGTACCAAAACTGAGTCTAATTTGGTTGATTGTCCCTTCAATGAACAGCC
  AGATCAGCAGAAGAGGGTCATCTGTAATTTCCAGATCAACGTTGCACCTTGGCTGAACAAGATGTCCATG
  ACAAACTTCAACTGTTACAATTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201025 representing BC048364
  Red=Cloning site Green=Tags(s)
  
  MASLLSPSMPVLAAVALTLTLAVIPEASTNAEAKQVVLGGVEPADPKDKEVQKVVKFAVRTYNDMDNDLY
  LSKPIRLMSASQQVVAGKNYYLKIELGRTTCTKTESNLVDCPFNEQPDQQKRVICNFQINVAPWLNKMSM
  TNFNCYNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048364
• ORF Size: 446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048364, AAH48364
• RefSeq Size: 740 bp
• RefSeq ORF: 446 bp
• Locus ID: 58214
• Cytogenetics: 2 G3
• Gene Summary: May play a role in the last steps of the chondrocyte differentiation pathway as an inducer of maturation (PubMed:13679380). Induces chondrocyte calcification during endochondral ossification by playing a role in the transcriptional inhibition of ENPP1, a generator of pyrophosphate which inhibits calcification (PubMed:16680148). Possibly impairs the binding of a transcription factor to the ENPP1 promoter (PubMed:16680148). Unlike other cystatins, does not have thiol protease inhibitor activity (PubMed:16680148).[UniProtKB/Swiss-Prot Function]