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Carhsp1 (NM_025821) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201012
Carhsp1 (tGFP-tagged) - Mouse calcium regulated heat stable protein 1 (Carhsp1)
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ORF Plasmid:
tGFP DDK
  $425.00
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Other products for "Carhsp1 or NM_025821"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Carhsp1
Synonyms 1200011K09Rik; Crhsp-24; D16Ertd465e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201012 representing NM_025821
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATCTGAGCCTCCTCCACCGCCGCTGCAGCCCCCCACGCATCAGACTTCTGTAGGGCTATTGGATA
CCCCTCGCACCCGGGATCGCTCTCCATCCCCCCTGCGGGGCAATGTGGTCCCTAGCCCTCTGCCTACCCG
CCGGACAAGGACCTTCTCAGCGACAGTGCGGGCTTCGCAGGGCCCCGTCTACAAAGGAGTCTGTAAATGC
TTCTGCCGGTCCAAGGGCCACGGCTTCATCACCCCAGCTGATGGTGGTCCTGACATCTTCCTGCACATCT
CTGATGTGGAAGGGGAGTATGTTCCGGTGGAAGGCGACGAAGTCACCTATAAGATGTGCTCTATCCCACC
CAAGAACGAGAAGCTGCAGGCTGTAGAGGTGGTCATCACCCACCTGGCACCAGGGACCAAGCATGAGACC
TGGTCTGGGCACGTCATCAGCAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201012 representing NM_025821
Red=Cloning site Green=Tags(s)

MSSEPPPPPLQPPTHQTSVGLLDTPRTRDRSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKC
FCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHET
WSGHVISN

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025821
ORF Size 444 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025821.2, NP_080097.1
RefSeq Size 2849 bp
RefSeq ORF 447 bp
Locus ID 52502
UniProt ID Q9CR86
Cytogenetics 16 4.26 cM
Summary Binds mRNA and regulates the stability of target mRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025821" in other vectors (4)
SKU Description Size Price
MC201031 Carhsp1 (untagged) - Mouse calcium regulated heat stable protein 1 (Carhsp1), (10ug) 10 ug
$225.00
MR201012 Carhsp1 (Myc-DDK-tagged) - Mouse calcium regulated heat stable protein 1 (Carhsp1) 10 ug
$225.00
MR201012L3 Lenti ORF clone of Carhsp1 (Myc-DDK-tagged) - Mouse calcium regulated heat stable protein 1 (Carhsp1) 10 ug
$525.00
MR201012L4 Lenti ORF clone of Carhsp1 (mGFP-tagged) - Mouse calcium regulated heat stable protein 1 (Carhsp1) 10 ug
$525.00

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