Ube2d2a (NM_019912) Mouse Tagged ORF Clone

CAT#: MG200985

Ube2d2a (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2D 2 (Ube2d2)

Product Images

Circular map for MG200985

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ube2d2a
• Synonyms: 1500034D03Rik; Ubc2e; ubc4; Ube2d2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200985 representing NM_019912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGACCTGGCTCGAGATCCCCCAGCACAGTGTTCAGCAG
  GTCCTGTTGGAGATGATATGTTTCATTGGCAGGCTACAATAATGGGGCCAAATGACAGCCCCTATCAGGG
  TGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCGCCTAAGGTTGCATTTACA
  ACAAGAATTTATCACCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGGTCACAGTGGT
  CTCCAGCACTAACTATTTCAAAAGTACTTTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGA
  TCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAA
  TGGACTCAGAAGTATGCGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200985 representing NM_019912
  Red=Cloning site Green=Tags(s)
  
  MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFT
  TRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIARE
  WTQKYAM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019912
• ORF Size: 2480 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019912.2, NP_064296.1
• RefSeq Size: 2410 bp
• RefSeq ORF: 444 bp
• Locus ID: 56550
• UniProt ID: P62838
• Cytogenetics: 18 B2
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.[UniProtKB/Swiss-Prot Function]