Ube2v2 (NM_023585) Mouse Tagged ORF Clone
CAT#: MG200961
- TrueORF®
Ube2v2 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2 variant 2 (Ube2v2)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_023585" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ube2v2 |
Synonyms | 1110021H13Rik; 4632410D19Rik; 5730524P06Rik; 6820402M05; AI848315; C81524; MMS2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200961 representing NM_023585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCTCCACAGGAGTTAAAGTTCCTCGTAATTTTCGCTTGTTGGAAGAACTTGAAGAAGGACAAA AAGGAGTAGGTGATGGTACTGTTAGCTGGGGCCTTGAAGATGATGAAGACATGACACTTACAAGGTGGAC AGGCATGATTATTGGGCCACCAAGGACAAACTATGAAAACAGAATATATAGCCTGAAAGTAGAATGTGGA TCTAAATACCCAGAAGCTCCTCCATCAGTTAGATTTGTAACAAAAATTAATATGAATGGGATCAATAATT CCAGTGGAATGGTGGATGCACGGAGCATACCAGTATTAGCAAAATGGCAAAATTCCTATAGCATTAAAGT CATACTTCAAGAGCTAAGACGTCTTATGATGTCCAAAGAAAATATGAAGCTTCCACAGCCTCCAGAAGGA CAGACGTACAACAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200961 representing NM_023585
Red=Cloning site Green=Tags(s) MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECG SKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVILQELRRLMMSKENMKLPQPPEG QTYNN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_023585 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023585.4, NP_076074.2 |
RefSeq Size | 4181 bp |
RefSeq ORF | 438 bp |
Locus ID | 70620 |
UniProt ID | Q9D2M8 |
Cytogenetics | 16 A1- A2 |
Gene Summary | Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207699 | Ube2v2 (untagged) - Mouse ubiquitin-conjugating enzyme E2 variant 2 (Ube2v2), transcript variant 1, (10ug) |
USD 165.00 |
|
MR200961 | Ube2v2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2 variant 2 (Ube2v2), transcript variant 1 |
USD 150.00 |
|
MR200961L3 | Lenti ORF clone of Ube2v2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2 variant 2 (Ube2v2), transcript variant 1 |
USD 450.00 |
|
MR200961L4 | Lenti ORF clone of Ube2v2 (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2 variant 2 (Ube2v2), transcript variant 1 |
USD 450.00 |
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