Kcne1l (NM_021487) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200930
Kcne1l (tGFP-tagged) - Mouse potassium voltage-gated channel, Isk-related family, member 1-like (Kcne1l)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcne1l
Synonyms 1500015C14Rik; Kcne5; Mink
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200930 representing NM_021487
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTGCAGCGAGAGCCAACGGCTGCAAACCCTCTTGAACCGCTTGCTGCTGGAGCTGCACCATCGTG
GCAACGCCAGCGGCCTGGGCATCGGAACCGGCCCGAGCATGGGCATGGGGGTCGTCCCTGACCCTTTCGT
GGGCCGCGAGGCGACCAGCGCCAAGGGTAACGACGCTTATCTCTACATCCTACTCATCATGATCTTCTAT
GCCTGCCTAGCCGGAGGCCTCATCCTGGCCTACACCCGCTCCCGCAAGCTCGTCGAGGCCAAGGATGAGC
CACCCCTGGCCTGTGTTGCTGAGCAGGAATGGGTCCCCGCTGCCATCGCATCTGCCGACCCGGAGAATGG
CCAGGGCTTGCTGGCTGAGGGCGGCCACCAGCTCGCAGCAGGGGCGCTGCCTGCGCTGGCTCAGGGCGCT
GAGAGGGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200930 representing NM_021487
Red=Cloning site Green=Tags(s)

MNCSESQRLQTLLNRLLLELHHRGNASGLGIGTGPSMGMGVVPDPFVGREATSAKGNDAYLYILLIMIFY
ACLAGGLILAYTRSRKLVEAKDEPPLACVAEQEWVPAAIASADPENGQGLLAEGGHQLAAGALPALAQGA
ERV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021487
ORF Size 429 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021487.1, NP_067462.1
RefSeq Size 1446 bp
RefSeq ORF 432 bp
Locus ID 66240
UniProt ID Q9QZ26
Cytogenetics X F2
Summary Potassium channel ancillary subunit that is essential for generation of some native K(+) currents by virtue of formation of heteromeric ion channel complex with voltage-gated potassium (Kv) channel pore-forming alpha subunits. Functions as an inhibitory beta-subunit of the repolarizing cardiac potassium ion channel KCNQ1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021487" in other vectors (4)
SKU Description Size Price
MC201692 Kcne1l (untagged) - Mouse potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene (Kcne1l), (10ug) 10 ug
$150.00
MR200930 Kcne1l (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene (Kcne1l) 10 ug
$289.00
MR200930L3 Lenti ORF clone of Kcne1l (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene (Kcne1l) 10 ug
$450.00
MR200930L4 Lenti ORF clone of Kcne1l (mGFP-tagged) - Mouse potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene (Kcne1l) 10 ug
$450.00

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