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Txnl4a (NM_025299) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200918
Txnl4a (tGFP-tagged) - Mouse thioredoxin-like 4 (Txnl4), transcript variant 1
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Txnl4a
Synonyms D18Wsu98e; Dim1; ENSMUSG00000057130; Txnl4; U5-15kD; U5-15kDa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200918 representing NM_025299
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTACATGCTTCCGCATCTGCACAATGGCTGGCAGGTAGACCAGGCCATCCTTTCGGAGGAGGACC
GCGTGGTCGTCATTCGTTTCGGACACGACTGGGACCCCACTTGCATGAAGATGGACGAGGTTCTGTACAG
CATCGCCGAAAAGGTTAAAAATTTTGCAGTTATTTATCTTGTGGATATTACAGAAGTGCCTGACTTCAAC
AAGATGTACGAGCTGTATGACCCCTGCACTGTCATGTTCTTCTTCAGAAACAAACATATCATGATTGATT
TGGGCACTGGCAACAACAACAAGATCAACTGGGCCATGGAAGACAAGCAAGAAATGGTTGACATCATAGA
GACCGTGTACCGTGGCGCCCGCAAAGGCCGGGGTCTGGTGGTGTCTCCCAAGGACTATTCTACCAAGTAC
AGATAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200918 representing NM_025299
Red=Cloning site Green=Tags(s)

MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPTCMKMDEVLYSIAEKVKNFAVIYLVDITEVPDFN
KMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWAMEDKQEMVDIIETVYRGARKGRGLVVSPKDYSTKY
RY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025299
ORF Size 426 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025299.4
RefSeq Size 4071 bp
RefSeq ORF 429 bp
Locus ID 27366
UniProt ID P83877
Cytogenetics 18 53.34 cM
Summary Plays role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes that are involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025299" in other vectors (4)
SKU Description Size Price
MC203182 Txnl4a (untagged) - Mouse thioredoxin-like 4A (Txnl4a), transcript variant 1, (10ug) 10 ug
$150.00
MR200918 Txnl4a (Myc-DDK-tagged) - Mouse thioredoxin-like 4A (Txnl4a), transcript variant 1 10 ug
$289.00
MR200918L3 Lenti ORF clone of Txnl4a (Myc-DDK-tagged) - Mouse thioredoxin-like 4A (Txnl4a), transcript variant 1 10 ug
$450.00
MR200918L4 Lenti ORF clone of Txnl4a (mGFP-tagged) - Mouse thioredoxin-like 4A (Txnl4a), transcript variant 1 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.