Pomp (NM_025624) Mouse Tagged ORF Clone

CAT#: MG200906

Pomp (tGFP-tagged) - Mouse proteasome maturation protein (Pomp)

Product Images

Circular map for MG200906

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pomp
• Synonyms: 2510048O06Rik; Ump1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200906 representing NM_025624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCCAGAGGTCTTGGGTCGGAGCTGAAGGACAGTATTCCAGTCGCGGAGCTCTCAGCCAGCGGGC
  CTTTCGAGAGTCACGATCTTCTCCGGAAAGGGTTTTCTTGTGTGAAAAATGAACTTTTGCCCAGTCACCC
  TCTCGAGTTATCAGAAAAAAACTTCCAGCTCAACCAGGACAAGATGAACTTTTCCACGCTGAGGAACATC
  CAGGGTCTGTTTGCTCCCCTGAAGTTACAGATGGAATTCAAGGCAGTGCAGCAGGTTCACCGTCTCCCGT
  TTCTCCCGAGCTCAAACCTCTCACTGGATATTTTGAGGGGCAACGATGAGACCATTGGTTTTGAGGATAT
  TCTTAATGATCCATCACAAAGTGAACTGATGGGTGAGCCCCACGTGATGGTGGAACATAAGCTGGGCTTG
  CTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200906 representing NM_025624
  Red=Cloning site Green=Tags(s)
  
  MNARGLGSELKDSIPVAELSASGPFESHDLLRKGFSCVKNELLPSHPLELSEKNFQLNQDKMNFSTLRNI
  QGLFAPLKLQMEFKAVQQVHRLPFLPSSNLSLDILRGNDETIGFEDILNDPSQSELMGEPHVMVEHKLGL
  L
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025624
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025624.3
• RefSeq Size: 735 bp
• RefSeq ORF: 426 bp
• Locus ID: 66537
• UniProt ID: Q9CQT5
• Cytogenetics: 5 G3
• Gene Summary: Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation (By similarity). Mediates the association of 20S preproteasome with the endoplasmic reticulum (By similarity).[UniProtKB/Swiss-Prot Function]