Ly6e (NM_008529) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200821
Ly6e (tGFP-tagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ly6e
Synonyms Ly67; RIG-E; Sca-2; TSA-1; Tsa1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200821 representing NM_008529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCCACTTCCAACATGAGAGTCTTCCTGCCTGTGCTGTTGGCAGCCCTTCTGGGCATGGAGCAAG
TTCATTCCCTGATGTGCTTCTCATGTACCGATCAGAAGAACAATATAAACTGCCTGTGGCCAGTTTCATG
CCAGGAGAAAGACCATTACTGTATCACGTTATCTGCCGCTGCGGGCTTTGGGAATGTCAACCTTGGCTAC
ACCCTGAACAAGGGCTGCTCCCCGATCTGCCCCAGTGAAAATGTCAATCTCAATCTCGGTGTGGCGTCCG
TGAACAGCTACTGCTGCCAAAGCTCCTTCTGCAACTTCAGCGCAGCTGGCCTCGGACTTCGTGCCAGTAT
CCCACTACTGGGCCTTGGACTCCTGCTTAGCTTGTTGGCTCTGCTGCAGCTGAGCCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200821 representing NM_008529
Red=Cloning site Green=Tags(s)

MSATSNMRVFLPVLLAALLGMEQVHSLMCFSCTDQKNNINCLWPVSCQEKDHYCITLSAAAGFGNVNLGY
TLNKGCSPICPSENVNLNLGVASVNSYCCQSSFCNFSAAGLGLRASIPLLGLGLLLSLLALLQLSP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008529
ORF Size 408 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008529.3, NP_032555.1
RefSeq Size 2278 bp
RefSeq ORF 411 bp
Locus ID 17069
UniProt ID Q64253
Cytogenetics 15 34.29 cM
Summary Involved in T-cell development (PubMed:8258699). Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response (PubMed:26276394).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008529" in other vectors (6)
SKU Description Size Price
MC204027 Ly6e (untagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e), transcript variant 2, (10ug) 10 ug
$150.00
MC207208 Ly6e (untagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e), transcript variant 2, (10ug) 10 ug
$165.00
MG217844 Ly6e (tGFP-tagged) - Mouse lymphocyte antigen 6 complex locus E (Ly6e) transcript variant 2, (10ug) 10 ug
$350.00
MR217844 Ly6e (Myc-DDK-tagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e), transcript variant 2 10 ug
$289.00
MR217844L3 Lenti ORF clone of Ly6e (Myc-DDK-tagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e), transcript variant 2 10 ug
$450.00
MR217844L4 Lenti ORF clone of Ly6e (mGFP-tagged) - Mouse lymphocyte antigen 6 complex, locus E (Ly6e), transcript variant 2 10 ug
$450.00

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