Insl5 (NM_011831) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200819
Insl5 (tGFP-tagged) - Mouse insulin-like 5 (Insl5)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Insl5
Synonyms RIF2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200819 representing NM_011831
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGCCCCACTCTTGCTCTGTTTCTCCTCTTAGTTCTGTTGGCTGTGGTGGAAGTAAGAAGCAGGC
AGACTGTGAAGCTCTGTGGCCTGGACTACGTGAGAACAGTTATCTACATCTGTGCCAGCTCACGGTGGAG
GAGACATCTGGAGGGGCATTTCCACTCTCAACAAGCTGAGACAAGAAACTACCTCCAGCTCCTAGACAGG
CACGAGCCATCCAAGAAAACTCTGGAGCACAGCCTTCCCAAGACGGATCTCTCAGGACAGGAGCTTGTTC
GAGATCCACAGGCACCCAAGGAAGGTCTTTGGGAACTGAAGAAGCACTCAGTGGTATCCAGACGAGATCT
GCAAGCTCTGTGCTGCAGGGAAGGCTGCTCCATGAAGGAACTCAGCACCCTCTGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200819 representing NM_011831
Red=Cloning site Green=Tags(s)

MKGPTLALFLLLVLLAVVEVRSRQTVKLCGLDYVRTVIYICASSRWRRHLEGHFHSQQAETRNYLQLLDR
HEPSKKTLEHSLPKTDLSGQELVRDPQAPKEGLWELKKHSVVSRRDLQALCCREGCSMKELSTLC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011831
ORF Size 405 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011831.2
RefSeq Size 713 bp
RefSeq ORF 408 bp
Locus ID 23919
UniProt ID Q9WUG6
Cytogenetics 4 47.27 cM
Summary May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011831" in other vectors (4)
SKU Description Size Price
MC200861 Insl5 (untagged) - Mouse insulin-like 5 (Insl5), (10ug) 10 ug
$150.00
MR200819 Insl5 (Myc-DDK-tagged) - Mouse insulin-like 5 (Insl5) 10 ug
$289.00
MR200819L3 Lenti ORF clone of Insl5 (Myc-DDK-tagged) - Mouse insulin-like 5 (Insl5) 10 ug
$450.00
MR200819L4 Lenti ORF clone of Insl5 (mGFP-tagged) - Mouse insulin-like 5 (Insl5) 10 ug
$450.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.