Fabp2 (BC013457) Mouse Tagged ORF Clone

CAT#: MG200767

Fabp2 (tGFP-tagged) - Mouse fatty acid binding protein 2, intestinal (cDNA clone MGC:18570 IMAGE:4218050)

Product Images

Circular map for MG200767

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fabp2
• Synonyms: Fabpi; I-FABP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200767 representing BC013457
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCGACGGCACGTGGAAAGTAGACCGGAACGAGAACTATGAAAAGTTCATGGAGAAAATGGGCA
  TTAATGTGATGAAGAGGAAGCTTGGAGCTCATGACAATCTGAAACTGACAATCACACAGGATGGAAATAA
  ATTCACAGTCAAAGAATCAAGCAACTTCAGAAACATTGATGTTGTGTTTGAGCTCGGTGTAAACTTTCCC
  TACAGTCTAGCAGACGGAACGGAGCTCACTGGGGCCTGGACCATTGAGGGAAATAAACTTATTGGGAAAT
  TCACACGTGTAGACAATGGAAAGGAGCTGATTGCTGTCCGAGAGGTTTCTGGTAATGAACTAATCCAGAC
  CTACACATATGAAGGAGTTGAGGCCAAGCGATTCTTTAAGAAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200767 representing BC013457
  Red=Cloning site Green=Tags(s)
  
  MAFDGTWKVDRNENYEKFMEKMGINVMKRKLGAHDNLKLTITQDGNKFTVKESSNFRNIDVVFELGVNFP
  YSLADGTELTGAWTIEGNKLIGKFTRVDNGKELIAVREVSGNELIQTYTYEGVEAKRFFKKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013457
• ORF Size: 398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013457, AAH13457
• RefSeq Size: 774 bp
• RefSeq ORF: 398 bp
• Locus ID: 14079
• Cytogenetics: 3 53.74 cM
• Gene Summary: The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake, transport, and metabolism. This protein functions within enterocytes, possibly to sense lipids as part of energy homeostasis. In humans polymorphisms are associated with increased fat oxidation and insulin resistance. In mice deficiency of this gene alters body weight in a gender-specific manner and causes hyperinsulinemia. [provided by RefSeq, Jan 2013]