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Mt1 (BC027262) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200758
Mt1 (tGFP-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mt1
Synonyms Mt-1; MT-I
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200758 representing BC027262
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCAACTGCTCCTGCTCCACCGGCGGCTCCTGCACTTGCACCAGCTCCTGCGCCTGCAAGAACT
GCAAGTGCACCTCCTGCAAGAAGAGTGAGTTGGGACACCTTGGGTGGCGGCTAAGGCTAGGGGCGGGGAA
CTCCTACAAAACTGGCTCTGAGAAATGTCCTTTGCTTCCCGGAGGCCATTGTATTGTCTCGGGGACAGAA
CTATACAGAGAACTATTTAAAAAAACCGAGGTCTTCTCTGTTGGGGACAGGAAGCAGAGGTCTTCAGCCA
GGCTGACCTCTTCCTCCTCCTTTCTAGGCTGCTGCTCCTGCTGTCCCGTGGGCTGCTCCAAATGTGCCCA
GGGCTGTGTCTGCAAAGGCGCCGCGGACAAGTGCACGTGCTGTGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200758 representing BC027262
Red=Cloning site Green=Tags(s)

MDPNCSCSTGGSCTCTSSCACKNCKCTSCKKSELGHLGWRLRLGAGNSYKTGSEKCPLLPGGHCIVSGTE
LYRELFKKTEVFSVGDRKQRSSARLTSSSSFLGCCSCCPVGCSKCAQGCVCKGAADKCTCCA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC027262
ORF Size 396 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC027262, AAH27262
RefSeq Size 673 bp
RefSeq ORF 398 bp
Locus ID 17748
Cytogenetics 8 46.34 cM
Summary Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC027262" in other vectors (4)
SKU Description Size Price
MC206843 Mt1 (untagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861), (10ug) 10 ug
$165.00
MR200758 Mt1 (Myc-DDK-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861) 10 ug
$289.00
MR200758L3 Lenti ORF clone of Mt1 (Myc-DDK-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861) 10 ug
$450.00
MR200758L4 Lenti ORF clone of Mt1 (mGFP-tagged) - Mouse metallothionein 1 (cDNA clone MGC:27821 IMAGE:3483861) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.