H2bu2 (NM_030082) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200662
Hist3h2ba (tGFP-tagged) - Mouse histone cluster 3, H2ba (Hist3h2ba)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol H2bu2
Synonyms 1500011O09Rik; AI413321; Hist3h; Hist3h2ba
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200662 representing NM_030082
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGAGCCTTCCCGCTCCACTCCCGCCCCGAAGAAAGGCTCCAAGAAAGCCATCACCAAGGCTCAGA
AAAAGGATGGCAAGAAACGCAAACGGGGCCGCAAGGAGAGCTACTCCATCTACGTGTACAAGGTGCTGAA
GCAGGTGCATCCGGACACCGGCATCTCGTCCAAGGCCATGGGCATCATGAACTCGTTCGTCAATGACATC
TTCGAGCGCATCGCCAGCGAGGCCTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACGTCCCGCG
AGGTGCAGACGGCCGTGCGCCTGCTGCTGCCCGGGGAGCTGGCCAAGCATGCCGTGTCCGAGGGCACCAA
GGCAGTCACCAAATACACCAGCTCCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200662 representing NM_030082
Red=Cloning site Green=Tags(s)

MPEPSRSTPAPKKGSKKAITKAQKKDGKKRKRGRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
FERIASEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030082
ORF Size 378 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030082.4
RefSeq Size 623 bp
RefSeq ORF 381 bp
Locus ID 78303
UniProt ID Q9D2U9
Cytogenetics 11 B1.3
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_030082" in other vectors (4)
SKU Description Size Price
MC203639 Hist3h2ba (untagged) - Mouse histone cluster 3, H2ba (Hist3h2ba), (10ug) 10 ug
$150.00
MR200662 Hist3h2ba (Myc-DDK-tagged) - Mouse histone cluster 3, H2ba (Hist3h2ba) 10 ug
$289.00
MR200662L3 Lenti ORF clone of Hist3h2ba (Myc-DDK-tagged) - Mouse histone cluster 3, H2ba (Hist3h2ba) 10 ug
$450.00
MR200662L4 Lenti ORF clone of Hist3h2ba (mGFP-tagged) - Mouse histone cluster 3, H2ba (Hist3h2ba) 10 ug
$450.00

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