H2bc21 (NM_178214) Mouse Tagged ORF Clone

CAT#: MG200660

Hist2h2be (tGFP-tagged) - Mouse histone cluster 2, H2be (Hist2h2be)

Product Images

Circular map for MG200660

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: H2bc21
• Synonyms: AV127319; H2b-613; Hist2h2; Hist2h2be; T25626
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200660 representing NM_178214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAACTGGCCAAATCTGCCCCGGCTCCCAAGAAGGGTTCCAAGAAGGCTGTCACCAAGGCGCAAA
  AGAAAGATGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCATCTATGTGTACAAAGTGCTGAA
  GCAGGTGCACCCGGACACCGGCATCTCGTCCAAGGCCATGGGCATCATGAACTCGTTTGTCAATGACATC
  TTTGAGCGCATAGCAAACGAGGCTTCTCGCCTGGCGCATTACAACAAGCGGTCTACAATCACATCGCGGG
  AGATCCAGACGTCGGTGCGCCTTCTACTGCCCGGAGAACTGGCCAAGCACGCCGTGTCGGAGGGCACCAA
  GGCCGTCACCAAGTACACCAGCGCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200660 representing NM_178214
  Red=Cloning site Green=Tags(s)
  
  MPELAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
  FERIANEASRLAHYNKRSTITSREIQTSVRLLLPGELAKHAVSEGTKAVTKYTSAK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178214
• ORF Size: 378 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178214.4, NP_835586.2
• RefSeq Size: 2618 bp
• RefSeq ORF: 381 bp
• Locus ID: 319190
• UniProt ID: Q64524
• Cytogenetics: 3 F2.1
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of a putative stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Aug 2015]