Phlda3 (NM_013750) Mouse Tagged ORF Clone
CAT#: MG200655
- TrueORF®
Phlda3 (tGFP-tagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_013750" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Phlda3 |
Synonyms | Tih1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200655 representing NM_013750
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCGGCGGCGACCGTGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGCGGCGGGCTGCTGCAGCTGT GGAAGCGGAAGCGCTGCGTGCTCACCGAGCGCGGGCTGCAGCTCTTCGAGGCCAAGGGCACGGGCGGCCG GCCCAAGGAGCTCAGCTTCGCCCGCATCAAAGCCGTGGAGTGCGTAGAGAGCACCGGGCGCCACATCTAC TTCACGCTAGTGACCGAAGGCGGGGGCGAGATCGACTTCCGCTGCCCCCTCGAAGACCCTGGCTGGAACG CTCAGATCACCCTGGGCCTGGTCAAGTTCAAGAATCAACAGGCCATCCAGACTGTGCGGGCCCGGCAGAG TCTTGGGACTGGGACCCTTGTGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200655 representing NM_013750
Red=Cloning site Green=Tags(s) MTAAATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGTGGRPKELSFARIKAVECVESTGRHIY FTLVTEGGGEIDFRCPLEDPGWNAQITLGLVKFKNQQAIQTVRARQSLGTGTLVS TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013750 |
ORF Size | 375 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013750.2 |
RefSeq Size | 1483 bp |
RefSeq ORF | 378 bp |
Locus ID | 27280 |
UniProt ID | Q9WV95 |
Cytogenetics | 1 59.26 cM |
Gene Summary | p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201656 | Phlda3 (untagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3), (10ug) |
USD 150.00 |
|
MR200655 | Phlda3 (Myc-DDK-tagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3) |
USD 225.00 |
|
MR200655L3 | Lenti ORF clone of Phlda3 (Myc-DDK-tagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3) |
USD 525.00 |
|
MR200655L4 | Lenti ORF clone of Phlda3 (mGFP-tagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3) |
USD 525.00 |
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