OriGene Logo

Bloc1s1 (NM_015740) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG200647
Bloc1s1 (tGFP-tagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 1 (Bloc1s1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Bloc1s1 or NM_015740"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bloc1s1
Synonyms AI839753; BLOS1; Gcn5l1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200647 representing NM_015740
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCCCGCCTGCTCAAAGAACACCAAGCCAAGCAGAACGAACGCAAGGAGCTGCAGGAGAAGAGGA
GGAGAGAAGCTATCGCTGCAGCGACCTGCCTGACCGAAGCGTTGGTGGATCACCTCAACGTGGGTGTGGC
CCAGGCCTACATGAACCAGAGAAAGCTGGACCATGAGGTGAAGACTCTACAGGTCCAGGCTGCCCAGTTT
GCCAAGCAGACAGGCCAGTGGATTGGAATGGTGGAGAACTTCAACCAGGCACTTAAGGAAATTGGGGATG
TGGAGAACTGGGCTAGGAGCATCGAGCTGGACATGCGCACCATTGCCACTGCGCTGGAATACGTCTACAA
AGGGCAGCTGCAGTCTGCACCATCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200647 representing NM_015740
Red=Cloning site Green=Tags(s)

MLSRLLKEHQAKQNERKELQEKRRREAIAAATCLTEALVDHLNVGVAQAYMNQRKLDHEVKTLQVQAAQF
AKQTGQWIGMVENFNQALKEIGDVENWARSIELDMRTIATALEYVYKGQLQSAPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015740
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015740.3
RefSeq Size 551 bp
RefSeq ORF 378 bp
Locus ID 14533
UniProt ID O55102
Cytogenetics 10 77.19 cM
Summary Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension (PubMed:16760431, PubMed:19546860, PubMed:21998198). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. The BORC complex is most probably associated with the cytosolic face of lysosomes, may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_015740" in other vectors (4)
SKU Description Size Price
MC201942 Bloc1s1 (untagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 1 (Bloc1s1), (10ug) 10 ug
$150.00
MR200647 Bloc1s1 (Myc-DDK-tagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 1 (Bloc1s1) 10 ug
$289.00
MR200647L3 Lenti ORF clone of Bloc1s1 (Myc-DDK-tagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 1 (Bloc1s1) 10 ug
$450.00
MR200647L4 Lenti ORF clone of Bloc1s1 (mGFP-tagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 1 (Bloc1s1) 10 ug
$450.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.