BC025446 (NM_146058) Mouse Tagged ORF Clone

CAT#: MG200638

BC025446 (tGFP-tagged) - Mouse cDNA sequence BC025446 (BC025446)

Product Images

Circular map for MG200638

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BC025446
• Synonyms: MGC29251
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200638 representing NM_146058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTTCTCATGCTACAAGGACCTGTGTGTTCATCCTTCTTGTGGCCCTATTGTGTGCAGAAGGAG
  CTCAGGGGCTGGAGTGCTACTGTTGCCTGGGGGTCCCACTTGAGACTTCCTGCAAAACAATGACCTGCCC
  CTACTCTGATGGATTCTGTGTTACTCAGGAAGAAGAAGTTATTGTGGACTCTCACACAATCAAAGTAAAG
  AGCAATCTCTGCCTTCCCATCTGCCCTACTAATCCTGAAACTACTGAGTTCCTGGGTAATGCTGTCAACG
  TGAAGACTTTCTGTTGCAAGGAAGACCTCTGCAATGCAGCAGTTCTCACTGAAGGCAGCACCTGGACCAT
  GGCAGGGGAGTCCTCATGGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200638 representing NM_146058
  Red=Cloning site Green=Tags(s)
  
  MDSSHATRTCVFILLVALLCAEGAQGLECYCCLGVPLETSCKTMTCPYSDGFCVTQEEEVIVDSHTIKVK
  SNLCLPICPTNPETTEFLGNAVNVKTFCCKEDLCNAAVLTEGSTWTMAGESSWK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146058
• ORF Size: 372 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146058.1, NP_666170.1
• RefSeq Size: 1511 bp
• RefSeq ORF: 374 bp
• Locus ID: 223631
• Cytogenetics: 15 D3