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Sncg (NM_011430) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200624
Sncg (tGFP-tagged) - Mouse synuclein, gamma (Sncg)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sncg
Synonyms C79089; persyn
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200624 representing NM_011430
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTCTTCAAGAAAGGCTTCTCCATTGCCAAGGAAGGTGTTGTGGGTGCTGTAGAAAAGACCAAGC
AGGGAGTAACGGAGGCAGCTGAGAAGACCAAGGAGGGGGTTATGTATGTGGGCACCAAAACCAAGGAGAA
CGTGGTACAAAGTGTCACCTCAGTGGCTGAGAAGACCAAGGAGCAGGCCAATGCCGTGAGTGAAGCTGTG
GTCAGCAGCGTCAACACAGTGGCCAACAAGACCGTGGAGGAGGCTGAAAACATCGTGGTCACCACCGGGG
TGGTGCGCAAGGAGGACTTGGAACCCCCTGCACAGGACCAAGAAGCCAAAGAGCAAGAGGAGAATGAAGA
GGCCAAGAGTGGAGAAGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200624 representing NM_011430
Red=Cloning site Green=Tags(s)

MDVFKKGFSIAKEGVVGAVEKTKQGVTEAAEKTKEGVMYVGTKTKENVVQSVTSVAEKTKEQANAVSEAV
VSSVNTVANKTVEEAENIVVTTGVVRKEDLEPPAQDQEAKEQEENEEAKSGED

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011430
ORF Size 369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011430.3
RefSeq Size 727 bp
RefSeq ORF 372 bp
Locus ID 20618
UniProt ID Q9Z0F7
Cytogenetics 14 20.81 cM
Summary Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011430" in other vectors (4)
SKU Description Size Price
MC204690 Sncg (untagged) - Mouse synuclein, gamma (Sncg), (10ug) 10 ug
$150.00
MR200624 Sncg (Myc-DDK-tagged) - Mouse synuclein, gamma (Sncg) 10 ug
$289.00
MR200624L3 Lenti ORF clone of Sncg (Myc-DDK-tagged) - Mouse synuclein, gamma (Sncg) 10 ug
$450.00
MR200624L4 Lenti ORF clone of Sncg (mGFP-tagged) - Mouse synuclein, gamma (Sncg) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.