Lsm10 (NM_138721) Mouse Tagged ORF Clone

CAT#: MG200601

  • TrueORF®

Lsm10 (tGFP-tagged) - Mouse U7 snRNP-specific Sm-like protein LSM10 (Lsm10)


  "NM_138721" in other vectors (6)

Reconstitution Protocol

USD 350.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Lsm10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200601 representing NM_138721
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACTGAGCCACTCGGTGAAGGAGCGAACTATTTCTGAGAACAGCCTGATCATCCTGTTGCAGGGCC
TCCAAGGCCAAATAACCACTGTGGACCTTCGGGATGAGAGTGTGGCCCGAGGACGCATTGACAACGTGGA
TGCTTTCATGAATATCCGCCTGGCCAATGTCACCTATACCGACCGCTGGGGACATCAGGTTGAGTTGGAT
GACCTTTTTGTGACCGGTCGTAACGTCCGATACGTCCATATCCCAGATGGTGTGGACATCACTGCTACTA
TTGAGCAGCAGCTGCAGATCATCCACCGTGTGCGCAACTTTGGTGGCAAGGGTCAAGGTCGTCGAGAGTT
CCCCTCCAAAAGGCCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200601 representing NM_138721
Red=Cloning site Green=Tags(s)

MALSHSVKERTISENSLIILLQGLQGQITTVDLRDESVARGRIDNVDAFMNIRLANVTYTDRWGHQVELD
DLFVTGRNVRYVHIPDGVDITATIEQQLQIIHRVRNFGGKGQGRREFPSKRP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138721
ORF Size 366 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138721.2, NP_620046.1
RefSeq Size 927 bp
RefSeq ORF 369 bp
Locus ID 116748
UniProt ID Q8QZX5
Cytogenetics 4 D2.2
Gene Summary Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing (By similarity). Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed (By similarity). Required for cell cycle progression from G1 to S phases (By similarity). Binds specifically to U7 snRNA (By similarity). Binds specifically to U7 snRNA (By similarity). Binds to the downstream cleavage product (DCP) of histone pre-mRNA.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.