1700081D17Rik (BC048621) Mouse Tagged ORF Clone

CAT#: MG200587

  • TrueORF®

1700081D17Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700081D17 gene (cDNA clone MGC:58705 IMAGE:6742909)


Reconstitution Protocol

USD 365.00

3 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol 1700081D17Rik
Synonyms PEBP-4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200587 representing BC048621
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATGAAGCTGGTCGCAGCAGCACTTTGCTTGAGCCTCCTTGCAGCCGGCCTGTGGGTGGGCCTCT
CATTGACAGCTGAGTCCATTGAAGAAGGGAAACCTGGAGGAGAGAAACCTGGAGGAGGGAAACCTGGAGG
CAGCGGACGAGGATGTTTTCTCCCACCACTGCCAAAAGAAGATGTTTCACTATGCAGGAACCTAGAAGTT
TTCTACATGGAGATGGGGAACATCAGCTGTAAAATTGTTCCTAAGTGCAATCTATACAGACAGAAGATCA
CGGCCTGGCAGGCGCCGATAGTCAAGTTCCACACGGCTTTGGATGTGAGTGAACTGGGTTGGCTGAAGGA
GAACGTGGGGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200587 representing BC048621
Red=Cloning site Green=Tags(s)

MTMKLVAAALCLSLLAAGLWVGLSLTAESIEEGKPGGEKPGGGKPGGSGRGCFLPPLPKEDVSLCRNLEV
FYMEMGNISCKIVPKCNLYRQKITAWQAPIVKFHTALDVSELGWLKENVGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC048621
ORF Size 365 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC048621, AAH48621
RefSeq Size 724 bp
RefSeq ORF 365 bp
Locus ID 73523
Cytogenetics 14 D2
Gene Summary Promotes AKT phosphorylation, suggesting a possible role in the PI3K-AKT signaling pathway.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.