Hcfc1r1 (NM_181821) Mouse Tagged ORF Clone

CAT#: MG200577

Hcfc1r1 (tGFP-tagged) - Mouse host cell factor C1 regulator 1 (XPO1-dependent) (Hcfc1r1)

Product Images

Circular map for MG200577

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hcfc1r1
• Synonyms: HPIP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200577 representing NM_181821
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCTGCAACAGCCCCTGGAGCGAGGTCCCCCAAGTCGGGACCCACGGGCCACCACTGGGGTAACTC
  GCGGCCTAAACGCCAGTCTTTCCCCCAGGGAACCTTTGCACAAGCAGTTCCTGTCTGAGGAGAACATGGC
  CACCCATTTCTCCCGACTCAGCCTACATAATGACCACCCCTACTGCAGCCCCCCTGTGACTTTTCCCGAA
  GCCCTGCCACCACTCAGGAGCCCTTGCCCAGAGCTGCTTCTCTGGCGCTATCCCGGGAGCCTGATTCCTG
  AAGCCCTCAGGCTGCTGAGGCTGGGGGATACCCCCAGTCCCTACTACCCTGCGTCCCCAGCTGGGGATAT
  CGTGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200577 representing NM_181821
  Red=Cloning site Green=Tags(s)
  
  MILQQPLERGPPSRDPRATTGVTRGLNASLSPREPLHKQFLSEENMATHFSRLSLHNDHPYCSPPVTFPE
  ALPPLRSPCPELLLWRYPGSLIPEALRLLRLGDTPSPYYPASPAGDIVEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181821
• ORF Size: 360 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181821.2
• RefSeq Size: 1243 bp
• RefSeq ORF: 363 bp
• Locus ID: 353502
• UniProt ID: Q9CYQ5
• Cytogenetics: 17 A3.3
• Gene Summary: Regulates HCFC1 activity by modulating its subcellular localization. Overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. HCFC1R1-mediated export may provide the pool of cytoplasmic HCFC1 required for import of virion-derived VP16 into the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]