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Polr3k (NM_025901) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200406
Polr3k (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Polr3k or NM_025901"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Polr3k
Synonyms 12.3kDa; 1500004O14Rik; AI196849; AU014893; C11; RPC10; RPC11
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200406 representing NM_025901
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTGTTCTGCCCGGGCTGCGGGAACGGACTCATTGTGGAGGAGGGGCAGCGTTGCCATCGCTTCG
CCTGCAACACTTGCCCCTACGTCCATAATATCACCCGTAAGGTAACAAACCGAAAGTATCCAAAGCTAAA
AGAAGTGGACGACGTGCTTGGTGGAGCAGCTGCATGGGAGAATGTGGACTCTACTGCAGAGCCATGTCCA
AAATGCGAACATCCCCGTGCCTACTTCATGCAGCTTCAGACGCGTTCTGCAGACGAGCCAATGACCACCT
TCTATAAGTGCTGCAATGCTCAGTGTGGACATCGCTGGAGGGAT

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200406 representing NM_025901
Red=Cloning site Green=Tags(s)

MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAEPCP
KCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_025901
ORF Size 324 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025901.3, NP_080177.1
RefSeq Size 2925 bp
RefSeq ORF 327 bp
Locus ID 67005
UniProt ID Q9CQZ7
Cytogenetics 2 H4
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025901" in other vectors (4)
SKU Description Size Price
MC205793 Polr3k (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k), (10ug) 10 ug
$300.00
MR200406 Polr3k (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k) 10 ug
$289.00
MR200406L3 Lenti ORF clone of Polr3k (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k) 10 ug
$450.00
MR200406L4 Lenti ORF clone of Polr3k (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k) 10 ug
$450.00

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