Guca2b (NM_008191) Mouse Tagged ORF Clone
CAT#: MG200368
- TrueORF®
Guca2b (tGFP-tagged) - Mouse guanylate cyclase activator 2b (retina) (Guca2b)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008191" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Guca2b |
Synonyms | AV066530; Gca; Gcap2; Ugn; urog |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200368 representing NM_008191
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAGAAGCCAACTGTGGGCTGCCGTCGTCCTGCTGCTGCTCCTGCAGAGTGCACAGGGTGTCTACA TCAAGTACCATGGCTTCCAAGTCCAGCTGGAATCAGTGAAGAAGCTGAATGAGTTGGAGGAGAAGGAGAT GTCCAATCCCCAGCCGCGGAGAAGTGGCCTCCTCCCTGCTGTGTGCCATAACCCAGCCTTGCCCTTGGAC CTCCAGCCTGTTTGTGCCTCCCAGGAAGCTGCCAGCACCTTCAAGGCCTTGAGGACCATCGCCACCGACG AATGTGAACTGTGTATAAATGTTGCCTGTACAGGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200368 representing NM_008191
Red=Cloning site Green=Tags(s) MSRSQLWAAVVLLLLLQSAQGVYIKYHGFQVQLESVKKLNELEEKEMSNPQPRRSGLLPAVCHNPALPLD LQPVCASQEAASTFKALRTIATDECELCINVACTGC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008191 |
ORF Size | 318 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008191.1, NP_032217.1 |
RefSeq Size | 590 bp |
RefSeq ORF | 321 bp |
Locus ID | 14916 |
UniProt ID | O09051 |
Cytogenetics | 4 55.48 cM |
Gene Summary | This gene encodes a member of the guanylin family and preproprotein that is proteolytically processed to generate a mature protein product. The mature protein product, known as uroguanylin, is an endogenous ligand for the guanylate cyclase-C receptor and may regulate salt and water homeostasis in the intestine and kidneys. Homozygous knockout mice for this gene exhibit impaired sodium chloride excretion and elevated arterial pressure. This gene is present in a gene cluster with a related guanylin family member on chromosome 4. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202104 | Guca2b (untagged) - Mouse guanylate cyclase activator 2b (retina) (Guca2b), (10ug) |
USD 150.00 |
|
MR200368 | Guca2b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 2b (retina) (Guca2b) |
USD 150.00 |
|
MR200368L3 | Lenti ORF clone of Guca2b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 2b (retina) (Guca2b) |
USD 450.00 |
|
MR200368L4 | Lenti ORF clone of Guca2b (mGFP-tagged) - Mouse guanylate cyclase activator 2b (retina) (Guca2b) |
USD 450.00 |
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