Lbh (NM_029999) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200356
Lbh (tGFP-tagged) - Mouse limb-bud and heart (Lbh)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lbh
Synonyms 1810009F10Rik; 6720416L16Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200356 representing NM_029999
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGTATATTTCCCCATTCACTGCTCTGACTATCTGAGATCGGCTGAGATGACCGAGGTGATGATGA
ACGCTCCATCCATGGAAGAGATTGGTCTCAGCCCCCGCAAGGATGGCCTTTCCTATCAGATCTTTCCGGA
CCCATCAGACTTTGACCGTTGCTGCAAGCTGAAGGACCGCCTGCCCTCCATAGTGGTGGAACCCACTGAA
GGGGAGGTGGAAAGCGGGGAGCTCCGGTGGCCTCCTGAGGAATTCCTGGTCCAGGAGGATGAGCAAGACA
ACTGTGAAGAGACAACAAATGAAAAGAAGGACCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200356 representing NM_029999
Red=Cloning site Green=Tags(s)

MSVYFPIHCSDYLRSAEMTEVMMNAPSMEEIGLSPRKDGLSYQIFPDPSDFDRCCKLKDRLPSIVVEPTE
GEVESGELRWPPEEFLVQEDEQDNCEETTNEKKDQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029999
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029999.4
RefSeq Size 3086 bp
RefSeq ORF 318 bp
Locus ID 77889
UniProt ID Q9CX60
Cytogenetics 17 E1.3
Summary Modulates the activity of key transcription factors involved in cardiogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029999" in other vectors (4)
SKU Description Size Price
MC201472 Lbh (untagged) - Mouse limb-bud and heart (Lbh), (10ug) 10 ug
$150.00
MR200356 Lbh (Myc-DDK-tagged) - Mouse limb-bud and heart (Lbh) 10 ug
$289.00
MR200356L3 Lenti ORF clone of Lbh (Myc-DDK-tagged) - Mouse limb-bud and heart (Lbh) 10 ug
$450.00
MR200356L4 Lenti ORF clone of Lbh (mGFP-tagged) - Mouse limb-bud and heart (Lbh) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.