Borcs7 (NM_025563) Mouse Tagged ORF Clone

CAT#: MG200354

2010012O05Rik (tGFP-tagged) - Mouse RIKEN cDNA 2010012O05 gene (2010012O05Rik)

Product Images

Circular map for MG200354

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Borcs7
• Synonyms: 2010012O05Rik; 4930569L17Rik; AI413851; AI467257
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200354 representing NM_025563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTGGGACGCCGGATTCGCAAGCCCGATTTGGTCAGTCCGTGAAGGGGCTTCTCACGGAGAAGG
  TGAACACCTGTGGCACCGATGTAATCGCGCTCACCAAGCAGGTGCTGAAAGGCTCGCGGAGCTCCGAGCT
  GCTGGGGCAGGCAGCTCGAAACATGGTACTACAGGAAGATGCCATCTTACACTCGGAAGATAGTTTAAGG
  AAGATGGCAATAATAACAACACACCTTCAGTACCAGCAAGAAGCTATTCAGAAGAATGTGGAACAGTCGC
  CTGACCTGCAGGACCAGTTGAGTCACTTACTGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200354 representing NM_025563
  Red=Cloning site Green=Tags(s)
  
  MATGTPDSQARFGQSVKGLLTEKVNTCGTDVIALTKQVLKGSRSSELLGQAARNMVLQEDAILHSEDSLR
  KMAIITTHLQYQQEAIQKNVEQSPDLQDQLSHLLK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025563
• ORF Size: 315 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025563.3, NP_079839.1
• RefSeq Size: 2176 bp
• RefSeq ORF: 318 bp
• Locus ID: 66439
• UniProt ID: Q9CRC6
• Cytogenetics: 19 C3
• Gene Summary: As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.[UniProtKB/Swiss-Prot Function]