2610030H06Rik (BC094639) Mouse Tagged ORF Clone
CAT#: MG200313
- TrueORF®
2610030H06Rik (tGFP-tagged) - Mouse RIKEN cDNA 2610030H06 gene (cDNA clone MGC:107029 IMAGE:1361961)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | 2610030H06Rik |
Synonyms | RP23-304N5.3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200313 representing BC094639
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGTCTTGATAAAGCAGCGCTCAATGCGCTGCAGCCTCCAGAGTTCAGAAATGAAAACTCTTTAG CTGCAACCCTGAAGACACTGCTGTTCTTCACAGCTTTAATGATCACGGTTCCTATCGGCTTGTATTTTAC AACGAAAGCTTATATATTTGAAGGTGCCCTTGGAATGTCTAATAGAGACAGCTATTTTTATGCCGCAATT GTTGCTGTTGTTGCTGTTCATGTGGTTCTGGCCCTGTTTGTCTATGTGGCCTGGAATGAAGGCTCACGAC AGTGGCGTGAAGGCAAACAGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200313 representing BC094639
Red=Cloning site Green=Tags(s) MERLDKAALNALQPPEFRNENSLAATLKTLLFFTALMITVPIGLYFTTKAYIFEGALGMSNRDSYFYAAI VAVVAVHVVLALFVYVAWNEGSRQWREGKQD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC094639 |
ORF Size | 305 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC094639, AAH94639 |
RefSeq Size | 520 bp |
RefSeq ORF | 305 bp |
Locus ID | 67048 |
Cytogenetics | X A7.3 |
Gene Summary | Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function] |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.