Timm10b (NM_019502) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200306
Timm10b (tGFP-tagged) - Mouse fractured callus expressed transcript 1 (Fxc1)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Timm10b
Synonyms Fxc1; Tim9b; Tim10b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200306 representing NM_019502
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGCAGCAGCAGCAACTGAGAAACTTGCGAGACTTCCTGTTGGTCTACAATCGGATGACAGAAC
TGTGTTTCCAGCGCTGTGTGCCCAGCCTGCACCACCGAGCTCTGGACGCTGAGGAGGAGGCCTGCCTGCA
CAGCTGTGCTGGGAAACTCATCCATTCTAACCACCGCCTCATGGCCGCTTACGTGCACCTCATGCCCGCC
CTGGTCCAGCGCCGCATCGCGGACTACGAGGCTGCCTCGGCCGCGCCAGGTATTCCTGCAGAACAGACCA
GAGACTCGCCATCAGGCAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200306 representing NM_019502
Red=Cloning site Green=Tags(s)

MEQQQQQLRNLRDFLLVYNRMTELCFQRCVPSLHHRALDAEEEACLHSCAGKLIHSNHRLMAAYVHLMPA
LVQRRIADYEAASAAPGIPAEQTRDSPSGS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019502
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019502.3
RefSeq Size 1013 bp
RefSeq ORF 303 bp
Locus ID 14356
UniProt ID Q9WV96
Cytogenetics 7 E3
Summary Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, it may act as a docking point for the soluble 70 kDa complex that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019502" in other vectors (4)
SKU Description Size Price
MC207282 Timm10b (untagged) - Mouse fractured callus expressed transcript 1 (Fxc1), (10ug) 10 ug
$165.00
MR200306 Timm10b (Myc-DDK-tagged) - Mouse fractured callus expressed transcript 1 (Fxc1) 10 ug
$289.00
MR200306L3 Lenti ORF clone of Timm10b (Myc-DDK-tagged) - Mouse fractured callus expressed transcript 1 (Fxc1) 10 ug
$450.00
MR200306L4 Lenti ORF clone of Timm10b (mGFP-tagged) - Mouse fractured callus expressed transcript 1 (Fxc1) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.