Lsm8 (NM_133939) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200273
Naa38 (tGFP-tagged) - Mouse LSM8 homolog, U6 small nuclear RNA associated (S
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lsm8
Synonyms 2010003I05Rik; AW214405; Naa38
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200273 representing NM_133939
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCTGCTTTGGAGAACTACATCAACCGGACTGTGGCTGTTATTACTTCTGATGGCAGAATGATTG
TGGGAACACTAAAAGGTTTTGACCAGACCATTAACTTGATTTTGGATGAAAGCCATGAGCGGGTGTTCAG
CTCTTCACAGGGAGTAGAACAAGTGGTGTTGGGGTTATACATTGTGAGGGGCGACAATGTAGCAGTCATT
GGGGAAATAGATGAAGAGACGGATTCTGCACTCGACTTGGGGAACATCCGAGCAGAGCCTCTGAACTCCG
TAGCACAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200273 representing NM_133939
Red=Cloning site Green=Tags(s)

MTSALENYINRTVAVITSDGRMIVGTLKGFDQTINLILDESHERVFSSSQGVEQVVLGLYIVRGDNVAVI
GEIDEETDSALDLGNIRAEPLNSVAH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133939
ORF Size 288 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133939.1, NP_598700.1
RefSeq Size 768 bp
RefSeq ORF 291 bp
Locus ID 76522
UniProt ID Q6ZWM4
Cytogenetics 6 A2
Summary Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133939" in other vectors (4)
SKU Description Size Price
MC203116 Naa38 (untagged) - Mouse N(alpha)-acetyltransferase 38, NatC auxiliary subunit (Naa38), (10ug) 10 ug
$150.00
MR200273 Naa38 (Myc-DDK-tagged) - Mouse N(alpha)-acetyltransferase 38, NatC auxiliary subunit (Naa38) 10 ug
$289.00
MR200273L3 Lenti ORF clone of Naa38 (Myc-DDK-tagged) - Mouse N(alpha)-acetyltransferase 38, NatC auxiliary subunit (Naa38) 10 ug
$450.00
MR200273L4 Lenti ORF clone of Naa38 (mGFP-tagged) - Mouse N(alpha)-acetyltransferase 38, NatC auxiliary subunit (Naa38) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.