Cox19 (NM_197980) Mouse Tagged ORF Clone

CAT#: MG200236

Cox19 (tGFP-tagged) - Mouse COX19 cytochrome c oxidase assembly homolog (S

Product Images

Circular map for MG200236

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cox19
• Synonyms: 1810074D02Rik; 2810437L13Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200236 representing NM_197980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACCGCAATGAACTTCGGGACCAAAAGCTTCCAGCCGCGGCCCCCAGACAAAGGCAGCTTCCCGC
  TGGACCACTTCGGTGAATGTAAAAGCTTTAAAGAAAAATTCATGAGGTGTCTCCGTGACAAGAACTATGA
  AAATGCTTTGTGCAGAAATGAATCTAAAGAGTATTTAATGTGCAGGATGCAAAGGCAGCTGATGGCACCG
  GAGCCGCTAGAGAAGCTCGGCTTTAGAGACCTAATGGAAGGGAAGCCAGAGGCAAAGGATGAATGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200236 representing NM_197980
  Red=Cloning site Green=Tags(s)
  
  MSTAMNFGTKSFQPRPPDKGSFPLDHFGECKSFKEKFMRCLRDKNYENALCRNESKEYLMCRMQRQLMAP
  EPLEKLGFRDLMEGKPEAKDEC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_197980
• ORF Size: 276 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_197980.1, NP_932097.1
• RefSeq Size: 778 bp
• RefSeq ORF: 279 bp
• Locus ID: 68033
• UniProt ID: Q8K0C8
• Cytogenetics: 5 G2
• Gene Summary: Required for the transduction of an SCO1-dependent redox signal from the mitochondrion to ATP7A to regulate cellular copper homeostasis. May be required for the assembly of mitochondrial cytochrome c oxidase.[UniProtKB/Swiss-Prot Function]