Hmgn2 (BC084680) Mouse Tagged ORF Clone

CAT#: MG200228

  • TrueORF®

Hmgn2 (tGFP-tagged) - Mouse high mobility group nucleosomal binding domain 2 (cDNA clone MGC:102472 IMAGE:6477991)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "BC084680" in other vectors (3)

Reconstitution Protocol

USD 365.00

3 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Hmgn2
Synonyms HMG-17; Hmg17
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200228 representing BC084680
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAAAGAAAGGCTGAAGGGGATGCTAAAGGAGACAAAACCAAGGTGAAGGACGAGCCACAGAGAA
GATCTGCAAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCAGAGCCCAAACCTAAAAAGGCCCCTGCGAA
GAAGGGAGAGAAGGTACCCAAGGGGAAGAAGGGGAAAGCTGACGCCGGGAAGGATGCGAATAATCCTGCA
GAAAATGGAGATGCCAAAACAGACCAGGCACAGAAAGCTGAAGGTGCTGGAGATGCCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200228 representing BC084680
Red=Cloning site Green=Tags(s)

MPKRKAEGDAKGDKTKVKDEPQRRSARLSAKPAPPKPEPKPKKAPAKKGEKVPKGKKGKADAGKDANNPA
ENGDAKTDQAQKAEGAGDAK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC084680
ORF Size 270 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC084680.1
RefSeq Size 1162 bp
RefSeq ORF 272 bp
Locus ID 15331
Cytogenetics 4 66.47 cM
Gene Summary Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.