Dbi (BC028874) Mouse Tagged ORF Clone

CAT#: MG200197

  • TrueORF®

Dbi (tGFP-tagged) - Mouse diazepam binding inhibitor (cDNA clone MGC:18631 IMAGE:4194069)


Reconstitution Protocol

USD 350.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Dbi
Synonyms ACBD1; Acbp; endozepine; EP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200197 representing BC028874
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAGGCTGAATTTGACAAAGCCGCTGAGGAGGTGAAGCGCCTCAAGACTCAGCCAACTGATGAAG
AGATGCTGTTCATCTACAGTCACTTCAAACAAGCTACCGTGGGCGATGTAAATACAGATCGGCCGGGGCT
CTTGGACCTCAAGGGCAAAGCCAAGTGGGACTCGTGGAACAAGCTGAAAGGGACTTCCAAGGAAAGTGCC
ATGAAGACCTATGTGGAAAAGGTAGACGAGCTAAAGAAGAAATACGGAATA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200197 representing BC028874
Red=Cloning site Green=Tags(s)

MSQAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESA
MKTYVEKVDELKKKYGI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028874
ORF Size 263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC028874, AAH28874
RefSeq Size 500 bp
RefSeq ORF 263 bp
Locus ID 13167
Cytogenetics 1 E2.3
Gene Summary Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.