Gng4 (NM_010317) Mouse Tagged ORF Clone
CAT#: MG200100
- TrueORF®
Gng4 (tGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 4 subunit (Gng4)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010317" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Gng4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200100 representing NM_010317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAGGCATGTCTAATAACAGCACCACCAGCATCTCCCAGGCCAGGAAAGCCGTGGAGCAGCTGA AGATGGAAGCCTGCATGGACAGGGTGAAGGTCTCCCAGGCTGCCTCAGACCTCCTGGCCTACTGTGAAGC CCACGTGCGGGAGGACCCCCTCATCATCCCAGTGCCTGCCTCAGAAAACCCCTTCCGGGAGAAGAAGTTC TTCTGCACCATCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200100 representing NM_010317
Red=Cloning site Green=Tags(s) MKEGMSNNSTTSISQARKAVEQLKMEACMDRVKVSQAASDLLAYCEAHVREDPLIIPVPASENPFREKKF FCTIL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010317 |
ORF Size | 225 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010317.3, NP_034447.1 |
RefSeq Size | 2976 bp |
RefSeq ORF | 228 bp |
Locus ID | 14706 |
UniProt ID | P50153 |
Cytogenetics | 13 5.29 cM |
Gene Summary | This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208572 | Gng4 (untagged) - Mouse guanine nucleotide binding protein (G protein), gamma 4 (Gng4), (10ug) |
USD 165.00 |
|
MR200100 | Gng4 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 4 (Gng4) |
USD 150.00 |
|
MR200100L3 | Lenti ORF clone of Gng4 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 4 (Gng4) |
USD 450.00 |
|
MR200100L4 | Lenti ORF clone of Gng4 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 4 (Gng4) |
USD 450.00 |
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